The Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.
The pIRES-hrGFP vectors contain a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES linked to the hrGFP coding sequence. This design allows the expression of a gene of interest to be monitored at the single-cell level due to expression of hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG epitope (pIRES-hrGFP-1a) or the HA epitope (pIRES-hrGFP-2a). Stable GFP-expressing cell lines are produced much more efficiently using Agilent’s hrGFP compared with EGFP.
For Research Use Only. Not for use in diagnostic procedures.