Member since: 2020
Organization: IRBM Science Park S.p.A
Great product for HTS application.
Application Area: Detection of ATPase activity
"Transcreener is a competitive immunoassay for ADP detection that uses TR-FRET technology. Good for HTS application."
The Transcreener® ADP² TR-FRET Assay produces a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) signal that is less sensitive to interference from fluorescent compounds than other detection modes. It is a simple, one-step homogeneous detection assay that accommodates a broad range of ATP concentrations (0.1 to 1,000 µM ATP) and produces asignal (Z´ > 0.7 at 10% conversion of 1 µM ATP). The assay can be used for virtually any ADP-producing enzyme including kinases and ATPases.
Displacement of tracer by ADP disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.