TaqPath™ 1-Step Multiplex Master Mix by Thermo Fisher Scientific
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Status:
Advanced Reviewer
Member since: 2014
Organization: Sanford-Burnham Medical Research Institute
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- Ease of use
- After sales service
- Value for money
The assay is very robust, we can save a lot of time and money.
Application Area: RT PCR for high Throughput Screening
"By using this one-step master mix, we can have up to 4 gene expression with 4 different dyes in one reaction. The assay is very robust, we can save a lot of time and money."
Review date: 17 Dec 2018 | TaqPath™ 1-Step Multiplex Master Mix
Status:
Advanced Reviewer Member since: 2014
Organization: Sanford-Burnham Medical Research Institute
-
- Ease of use
- After sales service
- Value for money
The best RT PCR multiplex Master Mix.
Application Area:RT PCR High throughput Screening
"We had tested different 1 step master mix from different vendors, the best one is TaqPath 1 Step Multiplex Master Mix. By using this master mix we can add up to 4 different gene expressions in one reaction. The result is very robust, it save time and money."
Review date: 21 Nov 2018 | TaqPath™ 1-Step Multiplex Master Mix
TaqPath™ 1-Step Multiplex Master Mix is a single-tube, 4X RT-qPCR master mix that provides for sensitive, reproducible detection of up to four different RNA (or DNA) targets in a single multiplex reaction. Particularly useful for RNA virus detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE™ dye (passive reference), and thermostable Fast DNA polymerase, all in a single tube.
This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample. With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix, is a superior choice for your multiplex diagnostic testing or development needs.
Features of the TaqPath 1-Step Multiplex Master Mix include:
- High sensitivity to detect low-copy targets with reproducible CT results
- Broad dynamic range with both RNA and DNA targets
- Optimized for multiplexing up to 4 targets simultaneously with MUSTANG PURPLE reference dye
- Tolerant of inhibitors commonly found in clinical samples
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