StemSpan™ T Cell Generation Kit has been developed to differentiate CD34+ cells isolated from cord blood (CB) to T cells, without use of stromal cells. This kit typically promotes the expansion of thousands of CD3+TCRαβ+ T cells in cultures initiated with CD34+ human CB cells. At 6 weeks, the majority of these cells are CD4+CD8+ double-positive (DP) cells, and can mature to CD8+ single-positive (CD8 SP) T cells after an additional week of stimulation.
StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion and differentiation of CD34+ cells isolated from human CB samples to CD7+CD5+ progenitor T (pro-T) cells. Additionally, StemSpan™ T Cell Progenitor Maturation Supplement (10X) enables further maturation of pro-T cells to DP cells. It also supports maturation of DP cells to CD8 SP T cells with additional stimuli. StemSpan™ Lymphoid Progenitor Expansion Supplement (10X) and StemSpan™ T Cell Progenitor Maturation Supplement (10X) are intended for use in combination with StemSpan™ SFEM II medium, and on plates coated with StemSpan™ Lymphoid Differentiation Coating Material (100X).
Manufacturer STEMCELL Technologies Inc.
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