RNAscope™ Multiplex Fluorescent Assays
The RNAscope Multiplex Fluorescent assays are ideal for simultaneous detection studies of any genes in nearly any tissue-type using fluorescent labels.
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Technical support team is nice and always there to help, Its good value for money
Detection of target RNA within intact cells.
Customer support is great. I had a tough time optimizing the assay due to the nature and type of my target tissues, but I got outstanding technical support from the ACD team during the standardization process. Results are reproducible if the quality of tissues is maintained. They have hundreds of ready-to-go probes so it saves a lot of time and effort to make your own probes. The detection method is very easy compared to the standard ISH. The protocol is easy to follow.
Review Date: 6 Oct 2021 | Advanced Cell Diagnostics
Great for brain tissue.
Analyze gene expression in brain samples
Easy to use and mostly works well. Found the best application in brain samples. It can be tricky to optimize for other tissues, tried adrenal and placenta as well and they neve work as well even after fiddling with many parameters.
Review Date: 24 Sept 2021 | Advanced Cell Diagnostics
So far good results. Go ahead if you are looking to buy it.
Detecting RNA using ISH
I have been using the RNAscope multiplex fluorescent V2 assay kit for few months now. The kit comes with a manual which guides you step by step through the protocol and it is very easy to use. Since I am new with this kit, I got the technical service package as well. The sales care and technical support team is very nice and always there to help, be it a phone conversation or a google team meet. Its good value for money except I feel that they should sell a few reagents like proteases and Amp buffers separately and not as a kit. Overall, I don't have any complaints.
Review Date: 6 Aug 2021 | Advanced Cell Diagnostics
Would recommend using RNAscope Multiplexing Fluorescent Assay for doing ISH.
Detecting RNA using ISH
The protocol is easy to follow. Customer support is great. The detection method is very easy with reduced background fluorescence compared to the standard ISH. They have hundreds of ready to go probes so it saves a lot of time and effort to make your own probes.
Review Date: 14 May 2021 | Advanced Cell Diagnostics
Great technology to detect gene targets where traditional IHC has been a challenge.
Gene expression in rodent brain samples
In-person technical support was available to guide the process from beginning to end. Many steps but protocol was clear and easy to follow.
Review Date: 18 Nov 2020 | Advanced Cell Diagnostics
One of the best techniques to visualize and quantify RNA molecules in a tissue section.
RNA in situ hybridization
I have used RNAscope V2 (fluorescent) and chromogenic in-situ hybridization assays for spatial detection of target RNA species in plants and animal tissue sections. This technique is amazingly WONDERFUL. Once optimized, this method is straightforward and very easy to use. After sales customer care is one of the best in industry. I had a tough time to optimize the assay due to the nature and type my target tissues, but I got outstanding technical support from ACD team during the standardization process. Results are reproducible if quality of tissues are maintained. Initial investment on RNAscope tools, kit and probe designing is a bit expensive but full value for money. Now RNscope is fully optimized in our lab and we are using it routinely.We published a modified protocol on it (doi: 10.1186/s13007-020-00614-4) and employ this technology for a few other projects too. We got beautiful pictures of multiple target RNAs as per our research objective and now many of our collaborators are interested in RNAscope technology.
Review Date: 25 Jun 2020 | Advanced Cell Diagnostics
The RNAscope Multiplex Fluorescent assays are ideal for simultaneous detection studies of any genes in nearly any tissue-type using fluorescent labels. We offer with various levels of plexing to:
- FIND cells of interest by using cell specific markers
- CONFIRM cells by simultaneous detection of additional targets
- CHARACTERIZE cellular heterogeneity of complex tissues by mapping and identifying multiple cell subtypes simultaneously
Spatial analysis of gene expression is an essential tool for comprehensive studies of complex, highly heterogeneous tissues, such as the brain, tumors, and developing organs. Traditional RNA-ISH can be challenging due to low sensitivity, inconsistent detection accompanied with limited plexing. RNAscope Fluorescent Multiplex assays provide exceptional sensitivity, allowing single-molecule detection of RNA targets at the single cell level.
The multiplex assays offer exceptional sensitivity and allow single-molecule detection of up to 12 RNA targets simultaneously. These assays offer a novel and proprietary method of in situ hybridization (ISH) to achieve single cell resolution for RNA detection in a multitude of slide mounted samples. The assays are compatible with various sample types such as fresh frozen, fixed frozen and FFPE.
- Single molecule RNA detection.
- Single cell resolution
- Sensitive
- Specific
- Spatial Context
