RNAscope technology enables the incorporation of spatial mapping and confirmation of gene signatures into single cell RNA sequencing workflows
14 Mar 2022In this application note, the diverse cell types in the mouse striatum that have been previously identified by scRNA-seq were confirmed and spatially mapped using the RNAscopeTM Multiplex Fluorescent assay and the RNAscopeTM HiPlex assay. The major and minor gene signatures identified by scRNA-seq, including discrete D1 and D2 medium spiny neuron (MSN) subtypes, were confirmed.
Further cellular heterogeneity within the MSN subpopulations was marked by a transcriptional gradient, which was spatially resolved with the RNAscopeTM technology.
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RNAscope™ HiPlex Assays
Advanced Cell DiagnosticsIntroducing RNAscope HiPlex assay to expand your in situ hybridization potential.
RNAscope™ Multiplex Fluorescent Assays
Advanced Cell DiagnosticsThe RNAscope Multiplex Fluorescent assays are ideal for simultaneous detection studies of any genes in nearly any tissue-type using fluorescent labels.
RNAscope™ Technology
Advanced Cell DiagnosticsIndependent of your genes of interest, thes easy RNA in situ hybridization assays use common reagents and protocols; providing universal assay conditions. All RNA-ISH kits contain reagents in a convenient ready-to-use (RTU) format, and chromogenic dyes are available to interrogate a single or two genes simultaneously. Most versatile are the RNAscope Multiplex Fluorescent Reagent Kits, enabling you to interrogate 1, 2 or 3 genes simultaneously.