Reconstructed Bone (r-Bone™) 3D Cell Culture Service, Acute Myelogenous Leukemia (AML) by zPREDICTA

Reconstructed Bone (r-Bone) platform offers 1-to-1 reconstruction of cellular and extracellular compartments of human bone marrow microenvironment. r-Bone preserves the architecture of human bone marrow and enables accurate evaluation of response to treatment. 

Unique Advantages:

• Clinically relevant system demonstrating high correlation with clinical outcomes (in a clinical study, 19 out 21 cases of response vs. resistance to treatment were predicted correctly).
• Supports evaluation of efficacy and toxicity in a single assay.
• Suitable for testing of immuno-oncology agents.
• Comprehensive system to ascertain mechanisms of drug resistance.

Features:

• Bone marrow-specific extracellular matrix (ECM) drives native organization of primary multiple myeloma cells ex vivo. 
• AML medium supplement enables reconstruction of a disease-specific mileu. 
• ong-term survival of primary patient cells (viability is maintained for >30 days as compared to 1-4 days in standard 2D culture or Matrigel).
• Maintains cellular heterogeneity of the primary human bone marrow.
• Suitable for evaluation of single agents and combination treatments.
• Compatible with multiple therapeutic modalities, e.g. small molecules, antibody-drug conjugates (ADC), immuno-oncology agents, including CAR-T cells.
• Compatible with standard readouts, such as FACs, imaging, bioassays, etc.