WAP four-disulfide core domain protein 2, epididymal secretory protein E4, WAP5
Human Embryonic Kidney 293 (HEK293) Cells
N-terminal 8x histidine tag
Molecular Mass (kDa)
The predicted size of HE4 protein is 12 kDa. The affinity purified recombinant human HE4 showed two bands: the major band is about 20 kDa, and the minor band is about 12 kDa. Both bands were confirmed by Western blotting using anti-HE4 antibody. The 20-kDa band may represent N-glycosylated form (Ronny Drapkin, et al. Cancer Res 2005; 65(6): 2162-9).
Lyophilized from a 0.2 µm filtered solution in PBS.
Determined by BCA protein assay kit (Thermo Scientific)
ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array
Stability & Storage
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.