Recombinant Human HE4 by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: 230-30001
Recombinant human HE4 protein, derived from transfected HEK293 cells


Recombinant Human HE4 by RayBiotech Inc. product image
Recombinant Human HE4

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Synonyms

WAP four-disulfide core domain protein 2, epididymal secretory protein E4, WAP5

Species

Human

Source

Expression System
Human Embryonic Kidney 293 (HEK293) Cells
Gene Symbol
WFDC2
Uniprot Accession #
NCBI Gene ID
10406
Expressed Region
Glu31-Phe124
Conjugation/Tag
N-terminal 8x histidine tag
Molecular Mass (kDa)
The predicted size of HE4 protein is 12 kDa. The affinity purified recombinant human HE4 showed two bands: the major band is about 20 kDa, and the minor band is about 12 kDa. Both bands were confirmed by Western blotting using anti-HE4 antibody. The 20-kDa band may represent N-glycosylated form (Ronny Drapkin, et al. Cancer Res 2005; 65(6): 2162-9).

Specifications

Format
Lyophilized powder
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS.
Concentration
Determined by BCA protein assay kit (Thermo Scientific)
Preservatives
None
Endotoxin Level
Not determined
Protein Type
Recombinant Protein

Recommended Applications

ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array

Stability & Storage

Shipping
Ice packs
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.