RapiDxFire™ Lyo-Flex 1-Step RT-qPCR 5X Master Mix

Name: RapiDxFire™ Lyo-Flex 1-Step RT-qPCR 5X Master Mix
SKU: RT-qPCR
Rating: 0 (0 reviews)

Designed with input from top molecular diagnostic assay manufacturers, the RapiDxFire Lyo-Flex 1-Step RT-qPCR 5x Master Mix supports the development of diagnostic devices and assays that meet healthcare provider and consumer demand for fast, accurate results. With consistent results in a liquid state at room temp or in a lyophilised state, this master mix will help you achieve predictable performance in unpredictable conditio…

0.0/5.0

|0 Reviews|Be the first to review this product

Buy Now

Place your order directly with the manufacturer.

Ease of Use

After Sales Service

Value for Money

Be the first to leave a review

Description

RapiDxFire Lyo-Flex stands out from competitor mixes with an ideal combination of features and benefits.

The unique heat-activated thermostable reverse transcriptase can withstand cDNA synthesis at high temperatures, enabling the detection of previously elusive viruses.
5x concentration creates an opportunity for higher test sensitivity while reducing waste.
The master mix can detect RNA and DNA templates, making it ideal for viruses as well as bacteria.
With an abundance of key PCR reaction components, this master mix supports the detection of multiple pathogens in a single reaction.
The master mix remains stable in a broad range of temperatures and conditions, giving you more flexibility in shipping, storage, and automation setup.
Lyophilize this master mix and bring PCR-based pathogen detection out of the lab.

Application eBookClinical Diagnostics

Simplifying the journey from molecular diagnostic assay development to commercialization

Molecular diagnostics (MDx) is rapidly advancing, with a growing array of DNA- and RNA-based tests now available to physicians, pharmacists, geneticists, research scientists, and other healthcare professionals.

As labs work with human and microbial genes, test developers must comply with clinical guidelines and regulations. Both established companies and start-ups face challenges, including meeting regulatory standards, ensuring quality, and managing supply chain issues. Early planning and robust partnerships are key to overcoming these obstacles.

This essential guide explores these challenges through detailed case studies. It covers everything from early development and assay formulation, to supply chain security, offering expert insights on successfully bringing PCR, next-generation sequencing (NGS), and other nucleic acid-based diagnostic tests to market.

30 Jul 2024

Application NoteLife Sciences

RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix

LGC demonstrates the analytical sensitivity and specificity of the RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix for both RNA and DNA genomes, its effectiveness at single-tube multiplexing in an MDx scenario, and the stability of the reaction components under various storage and handling conditions.

7 Aug 2022

Application NoteLife Sciences

Pre- and post-lyophilization performance of RapiDxFire qPCR 5X Master Mix GF

In this application note, Biosearch Technologies and BioAcumen Global have partnered to demonstrate the performance of RapiDxFire qPCR 5X Master Mix GF pre- and post-lyophilization, using an African Swine Fever Virus (ASFV) detection assay (designed by BioAcumen Global).

11 Mar 2022

VideoClinical Diagnostics

The future of diagnostics: How innovators in molecular techniques are shaping healthcare

From enzymatics to informatics, Hillary Sullivan, Vice President of R&D at LGC Biosearch Technologies, shares her thoughts on some of the latest advancements in molecular diagnostics. With insights from successful partnerships in healthcare, Sullivan provides her perspective on what the most promising areas of scientific advancements might hold for this rapidly developing field in the next 25 years.

30 Jul 2023

Editorial ArticleClinical Diagnostics

A revolution in diagnostics – The race for five-minute point-of-care PCR testing

Learn how UK-based diagnostics company, LEX Diagnostics, is working with LGC Biosearch Technologies to precipitate a step-change in PCR usability and speed

18 Jul 2022

Product Overview

RapiDxFire™ Lyo-Flex 1-Step RT-qPCR 5X Master Mix

LGC Biosearch Technologies

0.0/5.0

|0 Reviews

Buy Now

Links

LGC Biosearch Technologies Manufacturer Website

Featured products

Request Quote for All Products

FailSafe™ PCR System
LGC Biosearch Technologies
The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time. The system includes the FailSafe™ PCR Enzyme Mix and a meticulously tested set of 12 FailSafe™ PCR “PreMixes” that cover a matrix of enzyme-specific PCR conditions that are optimal for amplifying different sequences. Each FailSafe PCR PreMix contains dNTPs, an enzyme mix-optimized buffer, and one of the carefully selected levels of MgCl2 and FailSafe™ PCR Enhancer with Betaine.* Along with the FailSafe Enzyme Mix, at least one PreMix will contain the optimal PCR condition you need for successful and optimal PCR results with any template. The FailSafe™ PCR Enzyme Mix is a unique blend of thermostable enzymes that is capable of amplifying the most difficult DNA templates with extremely high sensitivity and high fidelity. Based on side-by-side experiments to determine nucleotide incorporation error rates, the nucleotide incorporation accuracy of the FailSafe Enzyme Mix is at least 3 times higher than Taq DNA polymerase. LGC Biosearch Technologies is the exclusive source for the Epicentre brand to scientists worldwide.
(2)
KASP™ Genotyping Reagents
LGC Biosearch Technologies
Unmatched quality, reliability and credibility have established KASP as the foundational SNP genotyping chemistry for the Agrigenomics industry.
(31)
sbeadex Nucleic Acid Extraction Kits
LGC Biosearch Technologies
Our proprietary sbeadex magnetic microparticles are designed to deliver high yields of high quality DNA, and have long enabled high throughput automated processing. sbeadex™ magnetic microparticles offer two key features which enable the extraction and purification of high quality DNA preparations: A novel two step binding mechanism via a patented adapter enables binding of nucleic acids more specifically. Final washes with pure water to deliver nucleic acids in high yield, purity and quality. Features: Development of customised protocols to your requirements Tailor-made extractions like modified lysis or eluation steps according to user requirements Extraction speed optimisation - quicker results Highly flexible batch sizes Technical advantages: Final washes with pure water to deliver nucleic acids in high yield, purity and quality No potential PCR inhibitors in wash buffers - e.g. organic solvents, salts, polyethyleneglycol Applicable for most popular robotic platforms (f.e. Hamilton, TECAN or KingFisher) Available sbeadex kits: sbeadex plant kit sbeadex blood kit sbeadex plasmid kit sbeadex forensic kit sbeadex tissue kit sbeadex livestock kit sbeadex pathogen kit The sbeadex technologies are suitable for high throughput automation and we have substantial experience developing sbeadex kits and protocols for use with a wide range of automation systems.
(0)
Kleargene™ Spin Plates for Nucleic Acid Extraction
LGC Biosearch Technologies
Kleargene spin plates utilize a glass fibre solid support and are suited for manual or automated DNA extractions from small samples. This includes samples such as plant seeds and tissue, animal tissue (rodent tails, etc...), blood, buccal swabs, buffy coat, saliva and more. Kits are available in both 96 and 384-well formats. The technology is optimally suited for nucleic acid extractions yielding between 100 ng to 10 µg of DNA.
(1)
oKtopure™ - Automated nucleic extraction platform
LGC Biosearch Technologies
The oKtopure™ is a fully automated nucleic acid extraction instrument that enables a new level of high-throughput processing and significant cost savings. This platform combines standardised high-throughput DNA or RNA extraction from a variety of sample types with the proprietary sbeadex™ magentic bead based extraction chemistry. The oKtopure is part of our on-going programme to provide customers with high productivity solutions, first developed and optimised for use within our service labs, where every day we deliver Extraction, Sequencing and Genotyping services. Key to the delivery of consistently high quality and high yield DNA preparations from the oKtopure is its ability to bind and hold the magnetic beads, and hence the DNA in place whilst transferring the buffers required for washing, binding and elution steps. The final aqueous wash used in the sbeadex extraction protocols enables the delivery of nucleic preparations ideal for downstream applications such as SNP genotyping, NGS and Sanger sequencing. Features: High throughput - capable of processing eight 96-well plates (768 samples) in approximately one-and-a-half hours Robust tip washing system reduces consumable costs by 50% or more Preloaded methods for different sample types – fully customizable Extraction expertise and support from scientists who use the instrument daily and have been carrying out high throughput extractions since 1996!
(4)
NGS Services
LGC Biosearch Technologies
Next-generation sequencing (NGS) technologies have dramatically reduced sequencing costs and led to novel approaches in many fields of biology.
(2)
Genespin Platform
LGC Biosearch Technologies
The Genespin™ platform completes the nucleic acid extraction range has been engineered to fulfill the growing demands for the highest throughputs. Speed is of the essence when it comes to DNA analysis of plant breeding populations; whole generations must be analysed for the presence or absence of desired biomarkers in time to enable the correct plants to be used in the next generation of breeding. This creates a demand for high-throughput DNA extraction from plant tissues, with resultant purified DNA of a suitable quality for PCR-based applications. The Genespin™ enables semi-automated but high throughput DNA extractions from plant tissues, and utilises the proprietary Kleargene™ extraction chemistry. The Kleargene spin plates utilise a glass fibre solid support inserted into a microtitre plate, in 96- and 384-well formats. To guarantee a throughput of more than 5000 extractions per day, the Genespin has been optimised to load the buffers into the spin columns without any cross-contamination. Kleargene kits are available in both 96-well and 384-well formats and are suitable for extraction of between 100ng and 5 µg DNA from plant material. In conjunction with our SNPline™, the Genespin platform provides DNA of a quality that is sufficient for downstream applications including PCR SNP genotyping including KASP, andarray technologies. Features: Enables high throughput DNA extraction: 8 x 96 plates/ hour. - 96 format: up to 5,500 samples per day. - 384 format: up to 20,000 samples per day Minimise consumable costs: in-built tip wash station ensures that only one set of tips is required per run Replicator function: the Genespin instrument can be used to prepare DNA dilution plates (96- and 384-well) and to stamp DNA into PCR plates Efficient use of laboratory space. - Small format: 170 cm x 68.5 cm x 65 cm Compatible with existing SNPline installations
(1)
KASP Genotyping Services
LGC Biosearch Technologies
This complete service package is a cost-effective way to genotype large sample and SNP numbers for plant, livestock, and aquaculture species.
(1)
Extraction Services
LGC Biosearch Technologies
The first step in any study investigating genetic differences in an organism of interest is the purification of DNA, and the nucleic acid extraction teams at LGC provide DNA extraction services to meet the demands of any project. Depending on project size, specific customer requirements and the biological background of the project, we can provide an optimised solution using any one, or a combination, of our propietary extraction technologies.
(0)
IntelliQube™ integrated PCR workflow
LGC Biosearch Technologies
Fully Automated PCR Setup, Amplification and Analysis System. The IntelliQube is the first fully automated and high-throughput instrument featuring seamless integration of liquid handling, thermal cycling, detection, and data analysis. The IntelliQube supports quantitative real-time PCR, end-point PCR, and isothermal chemistries, allowing you to realize the benefits of inline automation enabled by the innovative Array Tape™ consumable. IntelliQube leverages best-in-class multi-functional dispensing for high speed liquid handling in 384- or 768-well Array Tape™. Precise sample dispensing into Array Tape is handled by an interchangeable CyBi® FeliX 96- or 384-channel pipetting head. The IntelliQube supports inline amplification with a Peltier thermal cycling block designed specifically for Array Tape™. Due to a unique block design and surface geometry, both 384- and 768-well Array Tape are supported, without requiring a block change. The IntelliQube features an advanced optical system optimized for inline fluorescence detection in Array Tape. Filtered LEDs are optimized for a variety of commonly used fluorophores excited within the range of 480-620 nm. A high-resolution CCD camera supports detection of fluorescence intensity within 5 detection channels for dyes emitting within the range of 510-705 nm. Multiplex data capture for all 5 optical channels can occur in as little as 10 seconds. Benefits: Walk-away operation Increased efficiency of lab personnel Reduced chemistry costs Increased throughput Reduced consumable costs
(4)
BHQ™ Probes
LGC Biosearch Technologies
Dual-labelled probes featuring the original BHQ dye technologies
(7)
BHQnova™ Probes
LGC Biosearch Technologies
Double-quenched probes that offer improved quenching over longer probe sequences
(0)
BHQplus™ Probes
LGC Biosearch Technologies
Compact PCR probes for detecting difficult target sequences
(2)
Custom oligos
LGC Biosearch Technologies
Custom design your oligonucleotide according to your specifications. Our probes and primers are manufactured at unmatched quality under ISO 9001 and 13485 compliance. Whether you need RUO or GMP grade oligos, choose from a vast selection of modifications to customize your oligos for your application.
(14)
Stellaris RNA FISH
LGC Biosearch Technologies
RNA visualization method that allows simultaneous detection, localization, and quantification of individual mRNA molecules
(17)