QX200™ Droplet Digital PCR System

Droplet Digital™ PCR (ddPCR™) technology has advanced the absolute quantification of nucleic acids, becoming a reliable tool for clinical and translational research. Examine the application of ddPCR for DNA methylation detection, and explore workflow strategies, and key parameters for achieving sensitivity, linearity, and reproducibility in assay design.

In this application note, discover the advantages of implementing digital PCR (dPCR) to achieve sensitivity and quantification of DNA and RNA in samples of interest, overcoming the limitations of dPCR. Furthermore, learn more about how dPCR data quality is defined to ensure experiments yield reliable results that can translate into meaningful scientific conclusions.

The use of RNA as a therapeutic to effect clinically meaningful targets is an exciting new area of work with huge potential.

In this infographic, Bio-Rad provides an overview of how RNA can be used in a therapeutic application, some of the challenges associated with this new field, and how Droplet Digital™ PCR (ddPCR™) technology is helping address some of these challenges to fuel the RNA therapeutics revolution.

Developing effective and reproducible gene therapies requires the use of sensitive and robust testing methods, such as Droplet Digital PCR, to validate the quality of the therapeutic product. Bio-Rad's portfolio of ddPCR Viral Quantification solutions offers an accurate, precise, and robust method to measure the viral titer in a sample.

In this whitepaper, Martin Lock, Director, Process Development Gene Therapy Program, University of Pennsylvania, shares data comparing an AAV genome titer assay based on ddPCR with a standard and an optimized qPCR assay for the titration of both single-stranded (ss) and self-complementary (sc) AAV genomes.

In this application note, Bio-Rad discusses migrating assays from real-time quantitative PCR (qPCR) to Droplet Digital PCR (ddPCR). These two PCR methods determine the concentration of sample DNA using fundamentally different approaches and a bridging study between the two methods presents data demonstrating the differences and similarities to consider.

Droplet Digital polymerase chain reaction (ddPCR) was developed to provide high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications.

This application note shows how droplet digital PCR can be used to determine which alleles are located on the same chromosome, a process known as phasing. Unlike traditional phasing methods which are labor-intensive, low-throughput and limited in distance, Drop Phase is easy to perform, high throughput and works over large genomic distances.

Circulating-free tumor DNA (cfDNA) harboring the T790M can be detected at low concentrations using highly sensitive ddPCR technology. Download this abstract summary of ddPCR cfDNA analysis in non-small cell lung cancer patients in Brazil, where investigators have validated liquid biopsy analysis in early phase clinical studies.

Droplet Digital PCR can provide novel insights into nearly every discipline of molecular microbiology. Digital PCR provides unrivaled precision in qPCR applications. Additionally, partitioning of the sample into thousands of discrete droplets provides many other advantages including improved detection of rare targets through massive reductions in effective background. The flexibility to measure multiple targets independently or, alternatively, to identify linked targets, provides additional improvements for many applications including viral load, microbial quantification and identification, pathogen detection and microbial ecology.

Droplet Digital PCR (ddPCR) enables rapid quantification of genome editing events. In this guide, learn more about genome edit detection using digital PCR and find out how to design and order ddPCR assays to detect non-homologous end joining (NHEJ) and homology directed repair (HDR) edits for any target of interest on Bio-Rad’s easy-to-use Digital Assay Site. Use these assays to detect editing events with ultra-high sensitivity using minimal amounts of input DNA.

For more information on the benefits of genome edit detection with Droplet Digital PCR, click here>>

Regenerative medicine is transforming healthcare by using stem cells to grow tissues and organs for transplantation. Here, Dr. Fabian Suchy of Stanford University explains how his team is working to grow human organs from stem cells, the challenges of genomic precision, and why droplet digital PCR is a critical tool for ensuring quality and fidelity in these groundbreaking processes.

In this video, Bio-Rad provides tips and tricks used in ddPCR assay design. Watch now to explore topics including the availability of commercial PrimePCR ddPCR assays from Bio-Rad, common pitfalls, and recommendations for designing an effective ddPCR assay.

This video provides an overview of the approaches that Bio-Rad is using in the battle against COVID‑19. Here, subject matter experts discuss the latest testing methods for the disease, including the latest information on:

• Detection and diagnosis with Droplet Digital PCR (ddPCR)
• Determination of viral load and post-infection clearance via ddPCR
• Surveillance of immune response and immunity via serology testing

Dr. Ed. Shuuring, Professor and senior clinical scientist at the University Medical Center Groningen, explains how Droplet Digital PCR enables his team to work with low amounts of sample to obtain high sensitivity and quantify copy number mutations when working with liquid biopsies of cancer patients.

Clinical pharmacologist, Dr. Marzia Del Re, describes how she detects new mutations for drug resistance in liquid biopsies using Bio-Rad's Droplet Digital PCR system. The QX200TM ddPCRTM System, as Del Re explains, not only helps identify the mutation but also quantifies it.

Diabetes is one of the world’s most prevalent diseases, with estimates indicating that over four hundred million people worldwide are afflicted with either Type I or Type II diabetes. Professor Raghu G Mirmira, Indiana University School of Medicine, describes how his team is working to prevent people from developing the disease. They are primarily focused on the pathways of Type I diabetes, an autoimmune condition, which they hope to detect by identifying early disease biomarkers.

Watch this video to hear how the use of Bio-Rad workflow solutions has simplified the Pirogov Russian National Research Medical University's research into gene dosage imbalance in patients with Down's Syndrome (Trisomy 21). This research group has developed a cell-based model deriving from induced pluripotent stem cells with Trisomy 21. The use of the Bio-Rad Droplet Digital PCR allows the research group to produce accurate results, using only small amounts of the initial matrix of cells, enabling them to select the cell lines with the correct gene knock-out for their research.

Learn how liquid biopsies are replacing solid biopsies and are increasing the speed of cancer diagnosis, improving outcomes for patients. Also, find out how liquid biopsy can be used to quantify and monitor mutation, enabling rapid decision-making and treatment plans.

Carolyn Reifsnyder, Senior Marketing Manager, Bio-Rad Laboratories, Inc., introduces new assays for genome edit detection using the droplet digital PCR system as well as the new ddSEQ™ Single-Cell Isolator, at AACR 2017. She discusses how Bio-Rad’s droplet digital PCR has become a mainstay in the field of liquid biopsy and mutation detection due to the level of sensitivity and precision it can achieve.

In this interview filmed at AACR 2017, hear how researchers at the Dana-Farber Cancer Institute are using Droplet Digital PCR to detect key mutations in cell-free DNA from liquid biopsies, helping improve understanding of drug resistance development as well as inform treatment decisions.

Aggregate analysis of eight clinical trials of patients with advanced non-small cell lung cancer (aNSCLC) was supported by Biodesix, using Bio-Rad Droplet Digital PCR technology

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Validated detection of viral replication genes using Droplet Digital™ PCR during cell and gene therapy production

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Highly sensitive droplet digital PCR-based liquid biopsies can detect the smallest amounts of residual cancer DNA and help oncologists refine treatment decisions for a variety of cancers

ddPCR technology is recommended in the Centers for Disease Control and Prevention guidelines (CDC) for wastewater testing

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