Member since: 2010
Organization: City of Hope National Medical Center and Beckman Research Institute Department of Cancer Genetics and Epigenetics 1500 E Duar
Great results and cannot live without it.
Application Area: Mutagensis of DNA sequencing
"The reagents in the kits with good quality. High efficiency to get site-directed mutagenesis through PCR on target nucleotide."
The original QuikChange Site-Directed Mutagenesis Kits eliminate the need for subcloning into M13-based bacteriophage vectors and for ss-DNA rescue. This makes site-directed mutagenesis studies simple and reliable since it allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. The XL version of the kit is specialized for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates and features components specifically designed for more efficient DNA replication and bacterial transformation. The QuikChange Multi system allows mutagenesis at multiple sites (up to 5) in a single round, using a single oligonucleotide per site.
For Research Use Only. Not for Use in Diagnostic Procedures.