Prometheus Panta system for stability characterization of biologics by NanoTemper Technologies

5.0
/
5.0
  |  1 reviews

Collect data on thermal unfolding, particle sizing, and aggregation simultaneously throughout the entire thermal ramp for high-resolution, domain-specific stability characterization of biologics.


Prometheus Panta system for stability characterization of biologics by NanoTemper Technologies product image
Prometheus Panta system for stability characterization of biologics
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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


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    Reviewer
  • Member since: 2021

  • Organization: Leopold GmbH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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A perfect instrument.
Rating: 5.0

  • Application Area: Protein stability

"We use the Prometheus for protein stability testing. This has enabled us to develop new formulations for use in autoimmune diseases. Thank you, NanoTemper, for this really great instrument and the excellent service."

Review date: 08 Apr 2021 | Prometheus Panta system for stability characterization of biologics

Features:

  • High-resolution data that reveals liabilities in drug candidates
  • Domain-specific information about your candidate molecule when you measure thermal unfolding, particle sizing, and aggregation simultaneously throughout the entire thermal ramp
  • Flexible throughput and a few choices for sample handling

Parameters that Prometheus Panta measures

Dynamic Light Scattering (DLS)
SIZE ANALYSIS

  • Tsize (from growth of rH in cumulants fit)
    • Temperature at which average particle size begins to increase
  • Tscattering (from scattering intensity)
    • Fragments, PROTACs, ions, nanoparticles, peptides, and carbohydrates
  • Average scattering intensity
    • Identifies if concentration is too high or low for proper size distribution analysis

CUMULANT OR SIZE DISTRIBUTION/REGULARIZATION ANALYSIS

  • rH
    • Hydrodynamic radius shows size of particle in solvated state
  • PDI
    • Polydispersity index represents distribution of size populations

SELF-INTERACTION ANALYSES

  • kD
    • Diffusion interaction identifies onset of unfolding and impact on colloidal stability
  • D0 determined from the kD analysis
    • Theoretical diffusion constant at concentration = 0

nanoDSF
THERMAL UNFOLDING

  • Tm (for 330 nm, 350 nm, and ratio)
    • Melting temperature, or point at which 50% is unfolded
  • Tonset (for ratio)
    • Temperature at which unfolding begins
  • Ea (*parameter derived from data)
    • Activation energy of unfolding
  • Reversibility of unfolding
    • Point at which irreversibility happens when unfolding

Backreflection
AGGREGATION

  • Tturbidity
    • Onset temperature of aggregation

Product Overview

Prometheus Panta system for stability characterization of biologics by NanoTemper Technologies product image

Prometheus Panta system for stability characterization of biologics

Manufacturer NanoTemper Technologies

5.0 / 5.0 | 1 reviews