Prometheus Panta for biologics stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies

Manufacturer NanoTemper Technologies  |  Available Worldwide
5.0
/
5.0
  |  1 reviews

Prometheus Panta measures thermal unfolding, particle sizing, and aggregation simultaneously, throughout an entire thermal ramp, for high-resolution, domain-specific stability characterization of biologics. Find additional information about your biologics candidates such as molecular weight and self-association to predict aggregation propensity. Prometheus Panta combines DLS, SLS, nanoDSF, and backreflection technologies to give you a full picture of your candidate’s stability.


Prometheus Panta for biologics stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies product image
Prometheus Panta for biologics stability profiling with particle sizing and molecular weight determination
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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
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5 out of 5
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  • Member since: 2021

  • Organization: Leopold GmbH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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A perfect instrument.
Rating: 5.0

  • Application Area: Protein stability

"We use the Prometheus for protein stability testing. This has enabled us to develop new formulations for use in autoimmune diseases. Thank you, NanoTemper, for this really great instrument and the excellent service."

Review date: 08 Apr 2021 | Prometheus Panta for biologics stability profiling with particle sizing and molecular weight determination

Features

  • Monitor the stability profile of a wide range of protein-based biologic candidates, including monoclonal antibodies (mAbs), their derivatives (e.g. Fabs), enzymes, or receptor blockers
  • Domain-specific information about your candidate molecule when you measure thermal unfolding, particle sizing, self-association, and aggregation simultaneously throughout the entire thermal ramp
  • High-resolution data that reveals even subtle differences in candidate behavior, exposing potential liabilities in your drug candidates
  • Flexible throughput and a few choices for sample handling

Parameters that Prometheus Panta measures:

 

Dynamic Light Scattering (DLS)

 

SIZE ANALYSIS

 

Tsize (from growth of rH in cumulants fit): Temperature at which average candidate size begins to increase, indicating the beginning of the unfolding process

Average scattering intensity: Identifies if sample concentration is too high or low for proper size distribution analysis

CUMULANT OR SIZE DISTRIBUTION/REGULARIZATION ANALYSIS

rH: Hydrodynamic radius shows the size of your candidate in its solvated state

PDI: Polydispersity index, represents distribution of size populations and indicates if a sample preparation is pure and contains well-folded candidate proteins/mAbs

SELF-INTERACTION ANALYSES

kD: Self-interaction parameter, used to predict the propensity of a biologic candidate to aggregate once it is at concentrations required for clinical administration

D0 (*derived from kD analysis): Theoretical diffusion constant at concentration = 0, a ranking parameter for finding optimal formulation conditions for a candidate

 

nanoDSF

 

THERMAL UNFOLDING

 

Tm (for 330 nm, 350 nm, and ratio): Melting temperature, or point at which 50% of your protein is unfolded; higher Tm values indicate greater thermal stability

Tonset/Ton (for ratio): Temperature at which unfolding begins; higher Ton values indicate increased thermal stability, and it is considered favorable to have a sharper slope at the unfolding inflection point, which occurs when the Ton is close in value to the Tm

Ea (*derived from thermal unfolding data): Activation energy of unfolding, a parameter that informs on the stability of a protein in a given buffer environment

Reversibility of unfolding: Point at which aggregated candidate protein cannot be recovered and refolded without other chemical means

 

Backreflection

 

AGGREGATION

 

Tturbidity: Onset temperature of amorphous aggregate (>12.5 nm radius) formation

 

SLS

 

SIZING INFORMATION

 

Molecular weight: Average molecular weight of all particles in solution

SELF-ASSOCIATION AND AGGREGATION PROPENSITY

Tscattering: Temperature at which scattering intensity begins to change, indicating increased size and aggregation

B22: Second virial coefficient, used to extrapolate self-association behavior at higher concentrations such as those used for subcutaneous administration