Prometheus Panta for biologics stability profiling with particle sizing and molecular weight determination by NanoTemper Technologies
Prometheus Panta monitors the thermal stability, turbidity, and dispersity of a protein sample, and provides the highest-resolution, highest-quality data for ranking and selecting candidates. Prometheus Panta outputs critical quality parameters about your therapeutic proteins of interest, including: their hydrodynamic radius, molecular weight, self-association propensity, and deltaG of unfolding.
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We highly recommend the PANTA
Protein biochemistry
Prometheus PANTA has been a great asset in formulation for the optimisation of protein production and storage for our recombinant proteins.
Review Date: 22 Jul 2024 | NanoTemper Technologies
A perfect instrument.
Protein stability
We use the Prometheus for protein stability testing. This has enabled us to develop new formulations for use in autoimmune diseases. Thank you, NanoTemper, for this really great instrument and the excellent service.
Review Date: 7 Apr 2021 | NanoTemper Technologies
Prometheus Panta uses dye-free, buffer-independent measurements from nanoDSF (nano Differential Scanning Fluorimetry), DLS (Dynamic Light Scattering), SLS (static light scattering) and backreflection technologies to create high-resolution stability profiles of your samples. Get high-quality information about your biologics with a versatile multi-parameter stability characterization tool.
Prometheus Panta measures thermal unfolding, particle sizing, and aggregation simultaneously, throughout an entire thermal ramp for high-quality, domain-specific stability characterization of biologics and other protein products. To do this it uses nanoDSF, DLS, SLS, and backreflection technologies in parallel to give you a full picture of your candidate’s stability. Get the highest-resolution, most reliable stability data for full biophysical characterization of your therapeutic proteins.
Here’s what makes Prometheus Panta the perfect choice for biologics characterization:
- Label-free measurements in solution
- Measure intrinsic fluorescence of your proteins with dual UV system: 330 nm and 350 nm fluorescence detection (nanoDSF)
- Monitor amorphous protein aggregates with near-UV turbidity detection (backreflection)
- Obtain particle sizing and dispersity information with dynamic light scattering (DLS) measurements in the visible light range
- Isothermal hold, self-interaction analysis (B22 and kD), and chemical denaturation measurement modes for additional insights into your therapeutic protein's developability profile
Key Features:
- Adjustable throughput in a single instrument: Capillaries hold 10 µl of sample; up to 48 samples at a time with single capillaries or up to 24 samples in an easy-to-load capillary chip
- nanoDSF parameters: Tm, Tonset, reversibility of unfolding
- DLS/SLS parameters: Tscattering, Tsize, rH, PDI, kD, average scattering intensity, B22, molecular weight
- Backreflection parameters: Tturbidity (onset of turbidity, particles >12.5nm in radius)
- Temperature range: from 15 °C to 110 °C
- Straightforward handling: simple sample preparation and intuitive software user interface
