Status:
ReviewerMember since: 2021
Organization: Leopold GmbH
A perfect instrument.
Application Area: Protein stability
"We use the Prometheus for protein stability testing. This has enabled us to develop new formulations for use in autoimmune diseases. Thank you, NanoTemper, for this really great instrument and the excellent service."
Features
Parameters that Prometheus Panta measures:
Dynamic Light Scattering (DLS)
SIZE ANALYSIS
Tsize (from growth of rH in cumulants fit): Temperature at which average candidate size begins to increase, indicating the beginning of the unfolding process
Average scattering intensity: Identifies if sample concentration is too high or low for proper size distribution analysis
CUMULANT OR SIZE DISTRIBUTION/REGULARIZATION ANALYSIS
rH: Hydrodynamic radius shows the size of your candidate in its solvated state
PDI: Polydispersity index, represents distribution of size populations and indicates if a sample preparation is pure and contains well-folded candidate proteins/mAbs
SELF-INTERACTION ANALYSES
kD: Self-interaction parameter, used to predict the propensity of a biologic candidate to aggregate once it is at concentrations required for clinical administration
D0 (*derived from kD analysis): Theoretical diffusion constant at concentration = 0, a ranking parameter for finding optimal formulation conditions for a candidate
nanoDSF
THERMAL UNFOLDING
Tm (for 330 nm, 350 nm, and ratio): Melting temperature, or point at which 50% of your protein is unfolded; higher Tm values indicate greater thermal stability
Tonset/Ton (for ratio): Temperature at which unfolding begins; higher Ton values indicate increased thermal stability, and it is considered favorable to have a sharper slope at the unfolding inflection point, which occurs when the Ton is close in value to the Tm
Ea (*derived from thermal unfolding data): Activation energy of unfolding, a parameter that informs on the stability of a protein in a given buffer environment
Reversibility of unfolding: Point at which aggregated candidate protein cannot be recovered and refolded without other chemical means
Backreflection
AGGREGATION
Tturbidity: Onset temperature of amorphous aggregate (>12.5 nm radius) formation
SLS
SIZING INFORMATION
Molecular weight: Average molecular weight of all particles in solution
SELF-ASSOCIATION AND AGGREGATION PROPENSITY
Tscattering: Temperature at which scattering intensity begins to change, indicating increased size and aggregation
B22: Second virial coefficient, used to extrapolate self-association behavior at higher concentrations such as those used for subcutaneous administration
Manufacturer NanoTemper Technologies | Available Worldwide
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