Prometheus NT.Plex + RA for high throughput stability characterization by NanoTemper Technologies

The Prometheus NT.Plex measures thermal unfolding and turbidity in parallel using near-UV detection systems. With nanoDSF and backreflection technologies, researchers can make better decisions about the stability of their protein formulations while using small volumes of sample. Great for researchers doing HTP screening of small molecule thermal-shift assays, buffer formulations, or mutational analysis. The RA (robotic arm) enables you to prepare four 384-well plates (1536 samples) to be automatically measured by the system. With the NT.RA, improve your throughput without sacrificing data quality.

Key Features:

• Label-free measurements in solution
• Broad dynamic range, as low as 0.02 mg/ml to >200 mg/ml protein
• Measure intrinsic fluorescence of your proteins with dual UV system: 330 nm and 350 nm fluorescence detection
• Monitor amorphous protein aggregates with near-UV turbidity detection
• Capillaries hold 10 μl of sample; 24 capillaries mounted on a chip are automatically loaded with the robotic arm, enabling you to walk away while up to 1536 data points are collected
• nanoDSF parameters: T_m, T_onset, reversibility of unfolding
• Backreflection parameters: T_turbidity (onset of turbidity, particles >12.5nm in radius)
• Temperature range: from 15 °C to 95 °C (up to 110 °C with High Temperature Upgrade)
• Upgrade your instrument with software for chemical denaturation measurements (μG, c50)
• Maintenance-free instrument
• CFR 21 Part 11 compatibility
• Straightforward handling: simple sample preparation and intuitive software user interface for both small-scale and high-throughput experiments