Member since: 2018
Organization: University of copenhagen
We get great results on this instrument. Its a fundamental part of our research.
Application Area: Screen small-molecules and characterize their interactions (affinity, kinetics) to proteins
"The instrument is easy to use and requires little daily maintenance. Software for setting up protocols as well as data processing (QDat) is very intuitive, and still versatile. The support from Pall's sales persons, service engineers and application scientists is efficient, friendly and service-minded."
The Pioneer FE platform is based on surface plasmon resonance (SPR) for real-time, label-free measurements of biomolecular interactions. The system utilizes OneStep® Injection technology to diffuse the compound solution into a moving stream of buffer to create a concentration gradient during the injection, providing high quality kinetic data (ka, kd) and affinity measurements (KD). With this dynamic approach, an analyte titration of three orders of magnitude can be recorded in one continuous injection. This eliminates the need to prepare and inject a full dilution series required in other SPR instrumentation. Pioneer systems provide rapid affinity data directly from primary screens, enabling the prompt identification of lead candidates whilst significantly reducing the time and cost associated with secondary screening. This changes the paradigm for fragment screening in Fragment-based Drug Discovery (FBDD) as affinity (KD) analysis can be performed on primary screening data.