Pioneer FE by Sartorius Group

The Pioneer FE platform is based on surface plasmon resonance (SPR) for real-time, label-free measurements of biomolecular interactions. The system utilizes OneStep^® Injection technology to diffuse the compound solution into a moving stream of buffer to create a concentration gradient during the injection, providing high quality kinetic data (k_a, k_d) and affinity measurements (K_D). With this dynamic approach, an analyte titration of three orders of magnitude can be recorded in one continuous injection. This eliminates the need to prepare and inject a full dilution series required in other SPR instrumentation.  Pioneer systems provide rapid affinity data directly from primary screens, enabling the prompt identification of lead candidates whilst significantly reducing the time and cost associated with secondary screening. This changes the paradigm for fragment screening in Fragment-based Drug Discovery (FBDD) as affinity (K_D) analysis can be performed on primary screening data.

Key Benefits: 

• Make decisions early on with reliable affinity (K_D) and kinetic (k_d, k_a) data directly from the primary screen 
• High throughput: up to 768 samples in 24 hours in a single experiment 
• The only SPR instrument to perform fragment binding competition assays in a single injection 
• Normalize screening data across different sensor chips, days, users or instruments to compare an entire screen campaign and select actives from the global data set