Phos-tag acrylamide can be used for mobility shift detection of phosphorylated proteins using phosphate affinity SDS-PAGE. Phos-tag labelled acrylamide is incorporated into the gel and subsequently during electrophoresis serves to retard the progress of phosphorylated proteins through the gel enabling separation of phosphorylated and non-phosphorylated isomers.
Phos-tag™ agarose can be used to construct a phosphate affinity column enabling purification of phosphorylated proteins suitable for down-stream analysis such as mass spectrometry or SDS-PAGE.
Phos-tag™ biotin ligands can be used to detect phosphorylated proteins on PVDF membrane as an alternative to anti-phosphorylated antibodies. Unlike traditional immunodetection the membranes can be probed without blocking and phos-tag biotin is sensitive enough to detect phosphorylated protein at nanogram levels.Subsequently membranes can easily be stripped and reprobed with conventional antibodies if desired.
Mass Spectrometry Kit
The Phos-tag™ mass analytical kit can be used to provide rapid and sensitive matrix-assisted laser desorption/ionisation-time of flight mass spectrometry using natural zinc and stable isotopes 64Zn and 68Zn.