OneStep™ PCR Inhibitor Removal Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D6030
4.9
/
5.0
  |  8 reviews
The OneStep™ PCR Inhibitor Removal Kit is a fast, one-step procedure for removing PCR inhibitors such as polyphenolics, humic/fulvic acids, tannis, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.


OneStep™ PCR Inhibitor Removal Kit by Zymo Research product image
OneStep PCR Inhibitor Removal Kit
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Average Rating: 4.9
8 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2015

  • Organization: James Cook University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Removes inhibitors, retains yield.
Rating: 5.0

  • Application Area: Environmental DNA (eDNA)

"Environmental DNA is full of inhibitors but working on a rare species means that nucleic acid retention is really important. These columns are effective in removing inhibitors while also retaining yield more effectively than silica clean up columns. The protocol is less involved, saving time, and uses less harsh chemicals, reducing exposure. I also really appreciate that Zymo offer sample kits for their products, this has really helped me in the important optimisation stages of my workflows."

Review date: 16 Jul 2020 | OneStep™ PCR Inhibitor Removal Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Technology Evolving Solutions



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast, easy results for a good price.
Rating: 5.0

  • Application Area:Removal of inhibitors for RT-qPCR

"We are trying out new nucleic acid extraction protocols and PCR inhibition has been a growing problem. Someone recommended us these filters so we decided to try them. So far, they have been able to remove the inhibition from every sample we have run through them. The kit also takes less than 10 minutes to use, which is great."

Review date: 05 Jun 2020 | OneStep™ PCR Inhibitor Removal Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Tangled Bank Conservation



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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An integral part of our eDNA survey assay.
Rating: 4.7

  • Application Area:Remove inhibitors from eDNA samples

"The Zymo PCR inhibitor kit has provided itself an extremely useful tool to our lab. It has allowed for successful PCR amplification when the sample was previously inhibited. This has given us better results for our eDNA samples for qPCR, where they might previously been unusable. The kit comes in either a spin column or 96 plate format, both of which are easy to use and time efficient. Zymo Research has also answered any questions I've had regarding the kit in a rapid and easy to understand way. We will continue to use this great product as we are extremely happy with its results."

Review date: 05 Jun 2019 | OneStep™ PCR Inhibitor Removal Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Illinois Natural History Survey



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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PCR Inhibitor Removal Kit makes the impossible possible.
Rating: 5.0

  • Application Area:Clean up of environmental DNA extractions

"My lab has been using the Zymo PCR Inhibitor Removal kit for years now, and I'm always impressed with its ability to transform the most difficult samples (those that are high in tannins and other inhibitors) to ultimately allow us to detect rare, threatened, and endangered species from them. Fast, cheap, and user friendly, the PCR Inhibitor Removal Kit has been an outstanding product for us!"

Review date: 05 Jun 2019 | OneStep™ PCR Inhibitor Removal Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: ANSES



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast and easy way to reduce PCR inhibitors.
Rating: 5.0

  • Application Area:Food virology

"It add a quick and simple step to the ISO 15216 standard method for food samples extraction after viral RNA extraction. It reduces significantly the PCR inhibitors in red fruit samples."

Review date: 30 Aug 2018 | OneStep™ PCR Inhibitor Removal Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Hawaii at Manoa - Tropical Plant & Soil Sciences Department



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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This is truly something that is affordable and offers repeatable results!
Rating: 5.0

  • Application Area:Purifying plant genomic DNA for downstream PCR.

"I'm very impressed! Some gDNA samples that I had extracted via CTAB method yielded cloudy, brown DNA solutions. These would not amplify any DNA via PCR. However, after passing the cloudy, brown DNA solutions through the Zymo OneStep PCR Inhibitor Removal Kit, the eluted samples were clear and I was able to amplify my genetic regions via PCR. Thank you so very much for this wonderful kit! It's extremely easy to use and very worth the cost for the kit."

Review date: 29 Jul 2016 | OneStep™ PCR Inhibitor Removal Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Molecular Pathology Lab Network



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Got the job done!
Rating: 4.7

  • Application Area:DNA Cleanup

"We used the product for clearing melatonin out of DNA prior to sequencing. It did the job and saved a lot of samples."

Review date: 29 Jul 2016 | OneStep™ PCR Inhibitor Removal Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Oklahoma State University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great product for tricky eDNA samples!
Rating: 4.7

  • Application Area:Removal of humic acid from eDNA samples.

"This is by far the quickest, easiest way to remove inhibitory substances from eDNA samples. It is as simple as one spin and does not result in the loss of my eDNA. I can clean up all of my samples quickly and turn around and use them in a qPCR reaction. It's a wonderful product."

Review date: 26 Jul 2016 | OneStep™ PCR Inhibitor Removal Kit

The OneStep™ PCR Inhibitor Removal Kit contains all the components needed for efficient removal of contaminants that can inhibit downstream enzymatic reactions (e.g. PCR and RT) from DNA and RNA preparations. The column matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean-up is as simple as applying, spinning, and recovering a sample from the column.

Product Overview

OneStep™ PCR Inhibitor Removal Kit by Zymo Research product image

OneStep™ PCR Inhibitor Removal Kit

Manufacturer Zymo Research

4.9 / 5.0 | 8 reviews