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Olink® Explore 1536 by Olink Proteomics

Manufacturer Olink Proteomics
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Explore the low-abundant plasma proteome! Measure 1,536 proteins with minimal sample volume. Unparalleled specificity & sensitivity of PEA technology. Readout on NGS.


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Olink® Explore 1536
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Olink® Explore 1536 is a high-multiplex, high-throughput protein biomarker platform that uses Proximity Extension Assay (PEA) technology coupled to an innovative new readout methodology based on Next Generation Sequencing (NGS) using the Illumina® NovaSeq instrument.

PEA uses matched pairs of antibodies (Abs) attached to unique DNA oligonucleotides. When the correct pair simultaneously binds to a target protein, their DNA oligonucleotides hybridize and serve as a unique template for DNA polymerase-dependent extension. This provides a double-stranded DNA molecule for each detected protein, providing a unique barcode for high-sensitivity, high-specificity readout. The well-established Olink Target 96-panels use qPCR for the readout step, but marrying our elegant PEA technology with NGS for readout in Olink Explore provides several advantages:

  • 4-fold increase in multiplexing (from 96-plex to 384-plex)
  • 16-fold increase in the number of assays per run (from 96 to 1536)
  • 6.5-fold increase in throughput