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Status:
Reviewer
Member since: 2021
Organization: VUMC
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- Ease of use
- After sales service
- Value for money
Incredibly innovative PEA technology! Amazing results.
Application Area: Biomarker identification
"This product used state of the art technology to allow one drop of blood, less than 3 microliter, to provide information on the protein assays being measured. This allows for incredible insight into real-time human biology to help facilitate the development of more effective and targeted therapies. This in turn allows scientists/researchers to create new drugs while simultaneously continuing with clinical research/basic life science research for possible large-scale discoveries that will address the low abundant plasma proteome. "
Review date: 08 Apr 2021 | Olink® Explore 1536
Olink® Explore 1536 is a high-multiplex, high-throughput protein biomarker platform that uses Proximity Extension Assay (PEA) technology coupled to an innovative new readout methodology based on Next Generation Sequencing (NGS) using the Illumina® NovaSeq instrument.
PEA uses matched pairs of antibodies (Abs) attached to unique DNA oligonucleotides. When the correct pair simultaneously binds to a target protein, their DNA oligonucleotides hybridize and serve as a unique template for DNA polymerase-dependent extension. This provides a double-stranded DNA molecule for each detected protein, providing a unique barcode for high-sensitivity, high-specificity readout. The well-established Olink Target 96-panels use qPCR for the readout step, but marrying our elegant PEA technology with NGS for readout in Olink Explore provides several advantages:
- 4-fold increase in multiplexing (from 96-plex to 384-plex)
- 16-fold increase in the number of assays per run (from 96 to 1536)
- 6.5-fold increase in throughput
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Product Overview
