Oasis® Sample Extraction Products

Most clinical laboratories are aware of liquid chromatography-mass spectrometry (LC-MS) and its capabilities within the diagnostic environment. However, there are still burning questions that prevent clinicians from adopting this increasingly important tool.

In this eBook, we illustrate the importance of LC-MS in the clinical diagnostics hospital laboratory, outlining the technology available to support you throughout the LC-MS workflow and providing real-world case studies from leading experts in clinical chemistry and pathology.

Plus, explore the new video guide on how to maximize lab efficiency and achieve faster sample preparation to result with Water’s automated LC-MS workflow, designed to help you convert your sample data into valuable clinical knowledge.

In this application note, a comparison was performed between Oasis PRiME HLB SPE, SLE and LLE in both plasma and urine matrices for bioanalysis and forensic toxicology. In plasma, 22 comonly analyzed pharmaceuticals, steroids, and drugs of abuse were extracted using the three aforementioned methods and the results were compared. In urine, 23 drugs of abuse representing opioids, stimulants, benzodiazepines, and synthetic cannabinoid metabolites were tested for forensic toxicology analysis.

This booklet contains a compendium of application notes and methods demonstrating how the Waters' Oasis PRiME HLB cleans up challenging food samples for better analysis of contaminants.

CoQ10 is a unique hydrophobic molecule that is a key component in ATP synthesis. In this application note, the efficient extraction and analysis of CoQ10 from plasma samples is detailed.

In Veterinary medicine, xylazine is an FDA approved tranquilizer often used in combination with ketamine. The recreational use of both drugs is on the rise in a number of countries. Recently, the cocktail has been reported as a date rape drug resulting in ketamine being re-classified as a Schedule III drug in the US. the analysis of xylazine and ketamine in biological samples presents a number of challenges to analysts. Hyphenated chromatography platforms have enabled the sensitivity and efficiency to detect trace levels of analytes previously unachievable by traditional means. The only bottleneck now is the lengthy sample preparation time required, which will inevitably delay forensic investigations.

The QuEChERS method is highly effective for extraction of a wide range of pesticides from fruits and vegetables. After initial extraction, dispersive solid phase extraction (dSPE) is typically used for cleanup prior to chromatographic analysis. Among the interfering substances found in many vegetables, chlorophyll is particularly bad for gas chromatography and can quickly result in severe contamination of the column in use. This application note presents Oasis PRiME HLB Cartridges as an effective alternative cleanup method to dSPE with no loss of planar pesticides.

This application note demonstrates the need for robust and sensitive analysis of peptide species challenges, both through chromatographic separation and mass spectrometry. Recombinant human insulin and its analogs are perhaps the best known and most widely sold biotherapeutics. Historically, such biologics have been quantified using ligand binding assays. However, specifically in the case of insulin and analogs, these affinity-based assays lack standardization, are subject to matrix effects, and in some cases lack adequate specificity. The use of ionKey/MS facilitated a reduction in sample volume and concomitant increase in sensitivity for the quantification of human insulin and 5 important analogs.

The safe use of crop protection products is of paramount importance to the agricultural chemicals manufacturing industry. In this application note, ACQUITY UPC2 level enantioanalysis of five triazole fungicides and tandem quadrupole mass spectrometry were used for the trace in wheat grain and/or wheat straw. UltraPerformance Convergence ChromatographyTM (UPC2) applies the performance advantages of UPLC® to SFC, combining the use of supercritical CO2 with sub-2-μm particle columns.

The use of spot testing is the most known technique in forensic analysis for rapid results. For a complete legal identification, a more robust methodology is required, often LC-MS or LC-MS/MS. However, to achieve satisfactory results most often with trace level detection requirements, extensive and time consuming sample preparation protocols and required to reach sub ppb levels. This application note demonstrates the use of multi-dimensional chromatography as a micro extraction technique and sequential 2D extraction for the analysis.

This application note describes a highly specific and sensitive methodology for the quantification of desmopressin in plasma using solid phase extraction (SPE) followed by reversed-phase UPLC® coupled to tandem quadrupole mass spectrometry. Desmopressin is a peptide containing nine amino acids, a form of the normal human hormone arginine vasopressin that reduces urine production, and is most commonly prescribed for the treatment of diabetes insipidus or nocturnal enuresis. The low circulating levels of desmopressin requires a high-sensitivity assay to accurately define its pharmacokinetics in humans, especially pediatrics. The combination of solid-phase extraction methodology, UPLC chromatography, and Xevo TQ-S mass spectrometer has facilitated the development of an assay for desmopressin with a LLOQ of 1 pg/mL in plasma.

Watch this video to discover how the Oasis PRiME HLB System from Waters speeds up your Solid Phase Extraction (SPE) workflow. Kim Haynes, Senior Product Marketing Manager in the Consumables Business Unit at Waters Corp, explains how the innovative 3 step protocol reduces waste, saves valuable time and results in faster and cleaner samples. The Oasis PRiME HLB is ideal for bioanalysis, toxicology, and the screening of contamination in the food industry.

Select Science reports on the latest technologies from HPLC 2015

SelectScience attended this year's HPLC show to bring you the latest advances in high performance liquid chromatography.

New solid phase extraction sorbent's three-step process is 40% faster and samples are 70% cleaner than existing techniques