Monolith NT.Automated by NanoTemper Technologies

The Monolith NT.Automated measures biomolecular interactions via MicroScale Thermophoresis (MST) in a fully automated fashion. MST is a unique technology to quantify biomolecular interactions in an easy, rapid and accurate fashion. Interactions can be detected even without an increase in mass or size upon complex formation. This allows MST to sensitively measure fragment or small molecule binding to target proteins.

The Monolith NT.Automated is designed for high-throughput applications. The instrument can be integrated in a liquid handling platform to allow 8 hours of unsupervised data acquisition. Furthermore, the capillary format avoids any equilibration or washing downtime. Thus, the Monolith NT.Automated is the ideal instrument for screening and hit identification applications.

In addition, the Monolith NT.Automated can be equipped with up to two different detection systems. The instrument therefore offers a great versatility and combines truly label-free measurements and high-affinity applications.

Features:

Screening projects can be performed in an automated fashion, with the typical MST benefits:

• Low sample consumption < 3 µl sample as low as 10 pM concentration.
• Fast Measurement as fast as 10 seconds per data point.
• Dynamic Range pM to mM dissociation constants.
• No Downtime Instruments needs no calibration, equilibration or washing steps.

Maximum throughput for single-point measurements and dissociation constants:

• 96 compounds in 16 min with a protein consumption of 0.96 pg.
• 8 Kds in 16 min with a protein consumption of 0.12pg for each binding curve.

Applications:

Interactions between any kind of biomolecules, from single ions, fragments and small molecules up to high molecular weight complexes.

• Fragment and compound screening.
• Hit validation.
• High throughput screening applications.