Molecular Rulers by Bio-Rad

Manufacturer Bio-Rad
Molecular rulers are DNA standards with precisely defined size intervals between bands (DNA ladders). Bio-Rad provides three types of molecular rulers: standard, EZ Load, and AmpliSize rulers.


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Molecular Rulers
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Bio-Rad molecular rulers are DNA standards with precisely defined size intervals between bands (DNA ladders) for simplified estimation of the length of nucleic acids separated on agarose gels. Bio-Rad provides three types of molecular rulers: standard, EZ Load, and AmpliSize rulers. To ensure a precise measurement of the size of your samples, most of these DNA ladders include a visually distinct reference band.

Standard Molecular Rulers

Standard molecular rulers consist of precisely sized DNA and are provided ready for dilution. Each vial contains DNA for 100 applications. The advantages of standard molecular rulers include:

  • Visually distinct reference bands
  • Five size ranges to select from
  • No contaminating plasmid DNA

EZ Load Molecular Rulers

Ready-to-load EZ Load molecular rulers consist of precisely sized DNA blended with loading buffer for added convenience (prediluted to a concentration appropriate for most electrophoresis runs). Each vial contains DNA for 100 applications and includes an additional vial of 5x sample buffer for preparing your own samples. The advantages of EZ Load molecular rulers include:

  • No preparation — ready for direct loading on agarose or polyacrylamide gels
  • Visually distinct reference bands
  • Five size ranges to select from
  • No contaminating plasmid DNA

AmpliSize Molecular Ruler

The AmpliSize molecular ruler contains blunt-ended DNA of known sequence at 50% GC content. It has ten bands (at 50, 100, 200, 300, 400, 500, 700, 1,000, 1,500, and 2,000 bp). Each vial contains DNA for 50 applications.