MagPlex-Avidin® Multiplexing Microspheres

xMAP^® Technology uses color-coded microspheres to detect multiple analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Depending on your targets and the application, there are five different microsphere types to select from to produce the best experimental results.

MagPlex-Avidin^® are avidin-coupled magnetic polystyrene microspheres that have been dyed into 100 spectrally distinct sets or “regions”, allowing them to be individually identified by an xMAP instrument. In the multiplex assay environment the microspheres act as both the surface for the solution phase assay when reacted with a biotinylated ligand and the spectral identifier that the instrument detects. The open architecture of xMAP Technology enables users to build custom multiplex assays or select from a broad menu of commercially available kits.

Each MagPlex-Avidin microsphere has deglycosylated avidin covalently coupled to its surface. Theto the surface of the addressable microsphere. For a complete protocol, visit the Luminex website.

MagPlex-Avidin are ideal for coupling small molecules, like biotinylatese beads enable the user to quickly and easily design custom bead arrays, simply by reacting biotinylated target-specific ligand(s) with the MagPlex-Avidin microsphere; permanently linking the capture molecule(s) d peptides and offer:

• Avidin protein coated surface
• Near-covalent biotin-binding chemistry
• Available regions: 30 off the shelf (OTS), 70 additional regions built to order (BTO)
• Available concentration: 2.5 (millions/mL)
• Available vial sizes: 1 mL
• Compatible xMAP instruments: FLEXMAP 3D^®(30 OTS and 70 BTO regions), Luminex^® 100/200™ (30 OTS and 50 BTO regions), MAGPIX^® (30 OTS and 20 BTO regions)
• Additional xMAP microspheres: MagPlex^®, MagPlex-TAG™, MicroPlex^®, SeroMAP™