KAPA Library Quantification Kits by Kapa Biosystems, Inc.

Manufacturer Kapa Biosystems, Inc.  |  Available Worldwide
KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making i... Read more


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Variability in cluster density

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KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification.

Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries. The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.

Features:

Accurately quantify PCR-competent sequencing templates

  • qPCR only “counts” those library molecules that can be sequenced
  • More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources

Improve pooling for multiplexed sequencing

  • Library concentrations determined by qPCR are more reliable compared to other methods
  • The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts

Eliminate drift with carefully quality-controlled DNA Standards

  • Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards
  • KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time

Improve throughput with automation

  • Library quantification assay is compatible with 96- and 384-well format
  • Library dilution, reaction setup and data analysis can be automated for HTP pipelines

 

Applications:

  • Quantification of libraries prior to pooling for multiplexed sequencing
  • Quantification of individual libraries or library pools prior to cluster amplification or emPCR
  • Quantification of libraries prior to and after hybridization capture
  • Quality control, optimization and troubleshooting of library construction processes or workflows