The InterPlay Mammalian TAP System allows recovery of interacting proteins from mammalian cells. The method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP).
Tandem affinity purification (TAP) yields the tagged protein and interacting proteins using gentle washing and small molecule elution conditions. Protease digestion is unnecessary to recover intact interacting protein partners because SBP and CBP elute easily from their affinity resins, leaving your proteins intact.
For Research Use Only. Not for use in diagnostic procedures.