The Angiogenesis PrimeKit enables dynamic measurement of vascular tube formation and disruption in a relevant human primary cell, co-culture model.
The kit is suitable for testing novel test samples (e.g. small molecules, antibodies, siRNAs) for pro- or anti-angiogenic activity, and can also be used for target validation and exploratory studies.
Each kit includes one frozen vial of human GFP-labeled HUVECs, one frozen vial of NHDF, a 96-well plate, all media components and a detailed assay protocol. Once received, you can run the assay immediately or at the time of your choosing. Various growth factor supplements are also available to be purchased separately.
This co-culture model recapitulates all phases of the in vivo angiogenesis process, including cell proliferation, migration, morphogenesis, and anastomosis. Under basal conditions, the cultures exhibit a low level of capillary-like tube formation after 8-14 days. Inclusion of vascular growth factors such as VEGF, EGF, or bFGF produces robust tube formation, typically >5-fold over basal.