In-Fusion^® HD Cloning Plus PCR Cloning Kits by Takara Bio

PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus

In-Fusion PCR Cloning Kits enable sequence independent, directional, and seamless cloning of ANY PCR fragment-or multiple fragments-into ANY linearized vector at ANY locus in a single 15-minute reaction. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The efficiency of In-Fusion HD Cloning Kits is over 95% across a broad insert size range (0.5 to 15 kb inserts). The In-Fusion Enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15 bp overlap at their ends. This 15 bp overlap can be engineered by designing custom primers for amplification of the desired sequences. This method can be used to clone single or multiple fragments into a single vector without sub-cloning.

In-Fusion HD Plus is a complete cloning solution that includes all the reagents required for successful cloning including the fast and high fidelity CloneAmp HiFi PCR Premix, the ultra-high efficiency Stellar competent cells, and a choice of PCR clean-up or gel extraction kit for DNA purification, along with the In-Fusion HD enzyme premix and reagents for control experiments as standard.

Features:

• Sub-cloning is unnecessary—Clone any insert, into any location, within any vector
• Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb
• Seamless construction—Final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
• Flexibility to clone single or multiple fragments—Clone single or multiple DNA fragments simultaneously, in a single reaction
• Can be used to perform site-directed mutagenesis

Applications:

• PCR cloning
• HTP cloning
• Multiple fragment cloning
• Gene synthesis
• Gene design
• Mutagenesis
• Domain swapping
• Domain modification