ResourceLife Sciences
Cloning Blunt-End Fragments of Various Sizes
4 Feb 2015Takara’s Mighty Cloning Reagent Set (Blunt End) is designed to allow PCR products to be cloned into blunt-end vectors simply and quickly. Since PCR products with or without dA termini can be blunt-ended and phosphorylated at the 5’-ends in a single reaction, DNA fragments can be ready for ligation in a simple step. Pretreatment of PCR products to inactivate enzymes or remove unused dNTPs and primers is unnecessary. The cloning efficiency of the kit was demonstrated with PCR products that were used either directly or after gel purification.
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Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cloning