ImageXpress Micro 4 High-Content Imaging System

3D cell models are morphologically diverse with varying characteristics based on the cell type and the underlying research questions. In this application compendium, we bring you helpful case studies to perform high-content imaging on a range of 3D models and resolve common challenges experienced in 3D cell culture assays.

Learn how to capture in-depth and high-quality images of spheroids, stem cells, organs-on-chips and whole organisms and read case studies from the scientists developing 3D cell assays to delve into the complex biology of neurodegenerative disease, angiogenesis and tumor microenvironment.

The eBook covers 3D high-content imaging protocols for:

• Characterizing compound effects on 3D cells in extracellular matrix
• Screening cancer therapeutics in spheroids
• Morphological characterization of 3D neuronal networks in an organ-on-a-chip model
• Characterization of angiogenesis in an organ-on-a-chip model
• High-throughput imaging assays using zebrafish
• High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids
  

This application note demonstrates a method for cancer cell spheroid production, followed by protocols for staining and imaging of these spheroids for use in multi-parameter cytotoxicity studies.

Label-free cellular assays are required for a multitude of biological applications that monitor the cell number, proliferation, health, confluency, and cytotoxicity. These applications necessitate efficient and robust transmitted light (TL) imaging and analysis capabilities providing precise segmentation for quantitation of cells and assessment in variety of cell responses and morphologies. Additionally, the coupling of high-contrast transmitted light imaging and fluorescent labeling and imaging is essential to numerous cellbased assays.

Cardiac hypertrophy is a condition associated with many heart diseases such as myocardial infarction, ischemia, hypertension, valvular dysfunctions, and is also observed as a toxic side effect of environmental contaminants or pharmaceutical drug candidates. It is characterized by different cellular changes including increased cell size and enhanced protein synthesis. One of the classic biomarkers for hypertrophy is B-type natriuretic peptide (BNP), which is over produced in hypertrophic cardiac cells.

Recently, zebrafish-based screening has gained favor as an alternative to mammalian screening due to cost, throughput and reduced ethical concerns. Zebrafish are a useful model for drug development because of their high biological similarity to humans. Studies in ontogeny and organogenesis have shown that their primary organ systems are very similar to humans, and the synteny between zebrafish and Homo sapiens is as high as 70-80%.

Apoptosis is an important mechanism signaling programmed death of cells in normal processes such as embryonic development, as well as in diseases including cancer and neurodegenerative conditions. This flexible assay can be performed using an ImageXpress® Micro High Content Imaging System and MetaXpress® Analysis Software for calculating the incidence of apoptotic cells in a well.

Optimize your 3D assay and make your next breakthrough in cellular imaging - with intelligent, innovative imaging solutions.

This video introduces the ImageXpress Micro 4 confgurable widefield system by Molecular Devices. Explore and interact with your data using the new MetaXpress 3D analysis module, from basic assays to complex 3D structures across the plane.

In this guest editorial, explore how artificial intelligence is becoming a powerful tool for drug discovery

ImageXpress Nano System makes automated imaging accessible for every lab conducting cellular imaging research