Angiotensin, a key player in the renin-angiotensin system, is a peptide hormone that causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It is derived from the precursor molecule angiotensinogen produced in the liver.
Angiotensin II is formed from Angiotensin I, which is removed of two terminal residues by the enzyme Angiotensin-converting enzyme (ACE). Angiotensin II acts as an endocrine, autocrine/ paracrine, and intracrine hormone. Angiotensin II is degraded to angiotensin III by angiotensinases that are located in red blood cells and the vascular beds of most tissues. It has a half-life in circulation of around 30 seconds, while in tissue, it may be as long as 15-30 minutes.
The effect of obesity on Angiotensin II has recently been reported. Obese patients show heightened renal vasodilation to blockade of the renin-angiotensin system, suggesting deficits in vascular responses to angiotensin II. This may due to increased reactivity of renal vasoconstriction to ANG II.
Angiotensin II has been associated with a number of important physiological processes in heart, brain, adrenal gland and kidney. For cardiovascular effect, Angiotensin II is a potent direct vasoconstrictor, constricting arteries and veins and increasing blood pressure. It is also the most important Gq stimulator of the heart during hypertrophy. For neural effects, Angiotensin II increases thirst sensation (dipsogen) through the subfornical organ (SFO) of the brain, decreases the response of the baroreceptor reflex, and increases the desire for salt. It increases secretion of ADH in the posterior pituitary and secretion of ACTH in the anterior pituitary. For adrenal effects, Angiotensin II acts on the adrenal cortex, causing it to release aldosterone. For renal effects, Angiotensin II has a direct effect on the proximal tubules to increase Na+ absorption.
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
The capture antibody provided in this kit recognizes human, mouse and rat Angiotensin II.
Target Protein Information
Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin II (Ang II)]
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
Standard, Biotinylated Angiotensin II peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.