HoloMonitor^® M4 Live Cell Analysis System by Phase Holographic Imaging (PHI)

"The system is very easy to use and generates high-quality, reproducible results. Dedicated data analysis offers detailed information on cell proliferation frequency, cell division duration, cell morphology and cell motility to precisely assess additive toxicity."

"The HoloMonitor machine is efficient for operating up to 96 samples simultaneously. Holomonitor can provide high-quality images and time-lapse movies. We always get professional operation instructions and data analysis suggestions from the service team."

"I am using HoloMonitor system for analyzing migration, morphology, and cell growth of cells from cell lines and primary cells, and I am very happy with the imaging system and using it a lot in my research. It is easy to use and you can get amazing data: videos/images of the cells (2D, 3D, magnifications, etc) and a range of different parameters from one single experiment. I believe it is a big advantage that it is label-free and inside the incubator, making it a more stable, "natural" milieu and less interference with the cells, and also it enables long experiments. Great microscope!"

"This microscope is perfectly suited for our cell culture under flow application as well as many other types of experiments where tracking cell behaviour and motility in real-time is a must. Because it even provides morphology data in 3D we now have insights into what the cells are doing at a level which we simply could not achieve before. Highly recommended."

"The HoloMonitor M4 is a beneficial device for live-cell imaging, providing a lot of data. The device does not require large space and the software application is user-friendly, while recorded pictures look great. The good thing is that you can analyze your experiment using one software assay, and later, you can rerun the recorded data in another assay, which allows you to get the maximum from your experiment. I like it and would recommend it to all people doing a cytotoxicity assessment based on cell proliferation and optical thickness of the adherent unstained cells. Cell migration tracing is another application I like and recommend."

"I'm the principal investigator of a small research group at a public primarily undergraduate institution. Even considering our limited resources, I believe that the Holomonitor M4 is a fairly priced system and is definitely worth the investment. It has truly been and will remain a critical component of our research program. Our microscope lives directly in our cell culture incubator and allows us to see how various treatments affect our cells in real-time with three-dimensional single-cell resolution - it's amazing! The Holomonitor App Suite software is particularly powerful allowing us to quantify changes in cell morphology and movement. Importantly, I also think this instrument is great for education and my students are absolutely fascinated by the quality and beauty of the videos produced. This system is intuitive to operate and it is very effective in generating label-free long duration time-lapse videos. We dissociate complex tissues containing a mixed population of cells for culture, and with the Holomonitor M4 we are able to easily distinguish different cell types by their unique size, shape, and movement patterns. We can't wait to see what new biology we will discover with this innovative approach! I highly recommend it!"

"The HoloMonitor system is easier to use than I would have expected from a product with such a range, and the software makes it a breeze to produce and reproduce, high-quality analyses of cell growth. We discovered new avenues of investigation when we could gather such detailed reports on how cells behaved in real time. For too long, in vitro cell culture has had to gather evidence in the dark. Using the HoloMonitor lets us monitor our cells as closely as we wanted to, and showing others actual footage of our results was priceless!"

"The HoloMonitor gave us an amazing view into what was going on with our cells during incubation. We used it to watch cells settle to the bottom of 96-well plates after seeding. We found a temperature-dependence of the cell settling patterns in edge wells. We got beautiful movies of the cell motion, and the analysis software showed us the combined vectors of cell rolling in each well. The tools were not difficult to learn. It was amazing to think that we had been missing all of these things that happen every day in the lab to our cells. A wealth of information was revealed."

"We have studied cell morphology on non-adherent cells. It is a challenge to track cells in suspension, however, with the HoloMonitor system, we were able to take time-lapse images of cells trapped inside silicon (PDMS) microwells. Image segmentation is very efficient and allowed us to analyze the morphology of hundreds of cells in a label-free way. The analyse of untouched cells really benefits the label-free aspect, avoiding any interferences with any staining of any cell manipulation. This instrument is versatile and user-friendly."