The system comes with four excitation laser channels (635, 680, 750, and 790 nm) and is useful for a range of applications including oncology, inflammation, and cardiovascular disease. It is ideal for researchers who are well-versed in 3D fluorescence tomography and require the widest range of in vivo applications and imaging agents.
STEP 1: FMT Data Generation
FMT laser-driven transillumination generates paired absorption and fluorescence data maps throughout the animal.
The anesthetized mouse is comfortably placed in PerkinElmer's portable Animal Imaging Cassette. Imaging sessions are rapid (2-3 minutes per animal), animal handling is simple and the mouse remains stable and immobilized for consistent, repetitive imaging results (see image below).
STEP 2: FMT Normalization
Normalized fluorescence measurements generated by processing all paired absorption and fluorescence acquisition data.
FMT minimizes the distorting effects of in vivo tissue heterogeneity, taking into account surface boundaries, tissue spectral characteristics and wave-guiding effects to correct and normalize each fluorescent measurement.
Data normalized by FMT algorithmic models of photon transport in tissue.
STEP 3: FMT Reconstruction