FMT 4000 In Vivo Imaging System by PerkinElmer, Inc.

The system comes with four excitation laser channels (635, 680, 750, and 790 nm) and is useful for a range of applications including oncology, inflammation, and cardiovascular disease. It is ideal for researchers who are well-versed in 3D fluorescence tomography and require the widest range of in vivo applications and imaging agents.

STEP 1: FMT Data Generation

• Transillumination: FMT transillumination of animal using powerful 80 mW lasers
• Creating Baseline Absorption Profiles: Baseline absorption profiles of each mouse's biological composition are measured by a laser-light raster scan. Paired absorption and fluorescence data maps generated from 10,000 - 100,000 source detector laser projections.

FMT laser-driven transillumination generates paired absorption and fluorescence data maps throughout the animal.

The anesthetized mouse is comfortably placed in PerkinElmer's portable Animal Imaging Cassette. Imaging sessions are rapid (2-3 minutes per animal), animal handling is simple and the mouse remains stable and immobilized for consistent, repetitive imaging results (see image below).

STEP 2: FMT Normalization

Normalized fluorescence measurements generated by processing all paired absorption and fluorescence acquisition data.

FMT minimizes the distorting effects of in vivo tissue heterogeneity, taking into account surface boundaries, tissue spectral characteristics and wave-guiding effects to correct and normalize each fluorescent measurement.

Data normalized by FMT algorithmic models of photon transport in tissue.

STEP 3: FMT Reconstruction

• Fluorescence quantified to the picomole at each point in the subject including deep tissue targets
• Fluorescence measurements calculated throughout the user-selected regions of Interest (ROI)
• Data provided in universal formats for analysis (including DICOM), database building, and decision-making
• Easily create animated movies including dynamic presentation of quantification data for presentation
• Data and images easily exported for multi-modality image fusion
• Quantification is performed on all animals at any depth with equal accuracy and without the need for an artificial image atlas for referential quantification.