Exact FL by Axion BioSystems

The Exact FL is an automated, dual fluorescence cell counter with an expanded field of view and unmatched resolution and magnification power. Using an advanced CMOS-based optical system and an image analysis software powered by Artificial Intelligence (AI), the Exact FL calculates the exact number of cells in a sample and provides reliable assessment of key cellular parameters, including cell viability and transfection efficiency. The increased magnification and high resolution offer an unprecedented level of detail, allowing to distinguish between whole cells and cell debris and count even the smallest of mammalian cells (CAR-T cells, stem cells, PBMCs or splenocytes).

• State-of-the-art optics – a 6.4 MP CMOS camera combined with 10× magnification allow to accurately distinguish individual cells in clumps and count cells down to 4 µm in diameter
• AI-driven image analysis – cell counting is performed by highly trained pattern recognition software, minimizing user-to-user variability
• Large field of view & multi-count – by taking multiple images of the same sample at various slide positions, a larger portion of the available counting volume can be analyzed. Increased counting volume provides significantly lower coefficient of variation values, as compared to traditional image based cell counters
• Add-ons – the Exact FL is the first cell counter with an expandable application range, including an additional module for counting organoids
• Fastest autofocus - the Exact FL has the fastest autofocus system on the market that minimizes user inputs and variability

State-of-the-art optics

Count even the smallest of mammalian cells

Mammalian cells come in all shapes and sizes. The Exact FL has far better optics compared to conventional systems. Using the 6.4 MP CMOS camera combined with 10× magnification, the Exact FL can visualize and count cells down to 4 µm in diameter. With increased field of view and multi-count feature, it is also possible to take multiple snapshots of the same sample at various slide positions, which increases the accuracy and reproducibility of a cell count.

Dual fluorescence cell counting

Expand the possibilities of automated cell counting

Primary cell-based assays can significantly benefit from fluorescence cell counting due to the number of issues associated with detecting primary cells using colorimetric dyes (e.g. Trypan Blue). The Exact FL, fitted with red and green fluorescent channels, in combination with appropriate fluorescent dyes, can evaluate viability and concentration of primary cell samples with maximum accuracy and reproducibility.

High-performance AI algorithms

Minimize user-dependent variation

Manual cell counting introduces user-to-user variability to a scientific study. In addition, the process of manual cell counting is challenging and time-consuming, especially when working with cells that are prone to clumping. Equipped with a robust pattern recognition software and a declustering algorithm, the Exact FL detects resuspended single cells, as well as individual cells within cellular clusters with high accuracy and without any bias.

Innovative organoid counting software

Organoids are stem cell-derived 3D culture systems that have been used to study genetic disorders, infectious diseases and various types of cancer. To ensure consistency and reproducibility across multiple organoid-based experiments, it is critical to use a fixed concentration of organoids of similar size. Using the Organoid Counting add-on module, the Exact FL can analyze bright-field images to determine precise number and size of organoids in a sample.

Freedom to choose between reusable or disposable counting slides

Save money by using reusable counting slides

The Exact FL is compatible with reusable counting chambers, which makes the process of automated cell counting much more cost-effective. Customers that prefer working with disposable slides, can also use the Exact FL with a range of disposable cell counting chambers.

Automated software updates & secure cloud data storage

All devices are connected to the cloud environment. This means that the data generated by the Exact FL fluorescence cell counter is processed, analysed and stored in one accessible and secure place, protected by a multi-layered cyber security system. Novel software updates are also introduced via the Cloud. With only a few clicks our customers can download and install these add-ons to make their user experience even more seamless. Importantly, the added features will not affect consistency and reproducibility of acquired results.