Eclipse DualTec Field-Flow Fractionation System by Wyatt Technology Corp.

Manufacturer Wyatt Technology Corp.  |   Model: WECLDAQ / WECLDOR  |  Available Worldwide
  |  1 reviews
The ultimate separation system for proteins, polymers and nanoparticles.

Eclipse DualTec Field-Flow Fractionation System by Wyatt Technology Corp. product image
Eclipse DualTec Field-Flow Fractionation System
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Average Rating: 4.7
1 Scientist has reviewed this product

4 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money

  • Status:

  • Member since: 2020

  • Organization: Pace Analytical Life Sciences

  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The Eclipse DualTech brings our characterization to a whole new level.
Rating: 4.7

  • Application Area: Characterization of biological materials (VLPs, LNPs, etc.)

"The Eclipse AF4 module has been a great addition to our characterization techniques. AF4 helps us show our clients a more complete analytical picture of their material. The instrument and software are relatively easy to use and the training we received from Wyatt was great. Overall, I think this instrument and its accompanying softwares are extremely important to our projects in providing valuable data to our clients."

Review date: 27 May 2020 | Eclipse DualTec Field-Flow Fractionation System

Asymmetric-Flow Field-Flow Fractionation (AF4) is a uniquely versatile means of size-based separations, capable of fractionating proteins and aggregates, liposomes, emulsions, viral particles, polysaccharides, nanoparticles, polymer latex particles, colloidal soil suspensions, chemical mechanical polishing slurries, and many more - with no stationary phase and almost no shear. The Eclipse® DualTec® is the premier instrument for AF4 fractionation and characterization.

The DualTec model supports both Hollow-Fiber Flow FFF (HF5) and Asymmetric-Flow FFF (AF4) techniques. Both may be integrated into one instrument and coupled seamlessly to a DAWN® - the most advanced light scattering detector for determination of molar mass and nanoparticle size – creating an AF4/HF5-MALS system. The DualTec is offered in configurations suitable for aqueous, organic or metal-free solutions. It integrates with industry-leading HPLC modules. 


  • Separation range: 1 – 10,000 nm
  • Solvent compatibility: 3 models - aqueous (biocompatible) and organic solvents, and metal-free
  • Supported HPLC modules: The Eclipse AF4 integrates with Agilent, Thermo and Shimadzu pumps and autosamplers as well as degassers, online detectors and mass spectrometers
  • Supported separation channels: 2 sizes of standard-injection analytical AF4; hollow-fiber flow FFF cartridge; Mobility® electrical/AF4 module for zeta-potential analysis;
  • Software: AF4 and HPLC modules may be controlled from native HPLC software packages with plug-ins, or from the VISION® chromatography software suite which includes SCOUT DPS® for virtual method development and size analysis, and controls ASTRA® software for MALS/DLS analysis


  • High-resolution molar mass and size distributions of proteins, soluble and insoluble aggregates, natural and synthetic polymers, polymersomes, exosomes, liposomes, viruses, virus-like particles, metallic nanoparticles, quantum dots, drug-delivery and diagnostic nanoparticles, emulsions and many more
  • Analysis of fragile macromolecules or particles that are sheared on a size-exclusion column, sticky proteins that adhere to the stationary phase, or large, heavily-branched polymers that elute abnormally from GPC columns
  • Separation and characterization of trace nanoparticles from environmental matrices such as soil or seawater
  • Elemental analysis of nanoparticles, when coupled to ICP-MS
  • Detailed size-zeta potential distributions of nanoparticles
  • AF4 is recognized by the FDA and other regulatory agencies as an orthogonal technique to size-exclusion chromatography for analysis of biopharmaceutical aggregation.

Product Overview

Eclipse DualTec Field-Flow Fractionation System by Wyatt Technology Corp. product image

Eclipse DualTec Field-Flow Fractionation System

Manufacturer Wyatt Technology Corp.  |  Available Worldwide

4.7 / 5.0 | 1 reviews