Discovery C18 HPLC Columns

Antibiotic resistance has become a major clinical problem so there is an urgent need to develop agents that act via novel mechanisms. In this application note, Bacitracin A and Virginiamycin M1 were analyzed by HPLC, using Discovery C18, Discovery C8, and Discovery RP-AmideC16 columns. These columns offer excellent resolution for this application.

There are more than 100 antibiotics on the market today, and many more are in development. Resistance to antibiotics is a significant problem; an antibiotic that takes a decade to bring to market can induce resistance within months of its introduction into clinical practice. Therefore, it is critical to monitor the level of antibiotics given to humans and animals and HPLC is a powerful tool for isolation and quantification of such. In this application, five tetracycline antibiotics were analyzed by HPLC, using Discovery C18, Discovery C8, and Discovery RP-AmideC16 columns.

Manufacturers routinely analyze vitamins in their products to determine quantitative levels and to monitor changes that have occurred in processing. HPLC is the preferred technique for vitamin separation because of its high selectivity. In this application note, water-soluble vitamins were separated by reversed-phase HPLC on a Discovery C18 column.

Food manufacturers routinely analyze vitamins in their products to determine quantitative levels and to monitor changes that have occurred in food processing. HPLC is preferred for vitamin separation because of its high selectivity. In this application note, various mixtures of fat-soluble vitamins in A, D, and E groups were studied by reversed-phase HPLC using Sigma-Aldrich Supelco Discovery columns.

There are more than 100 antibiotics on the market today, and many more are in development. Resistance to antibiotics is a significant problem; an antibiotic that takes a decade to bring to market can induce resistance within months of its introduction into clinical practice. Therefore, it is critical to monitor the level of antibiotics given to humans and animals and HPLC is a powerful tool for isolation and quantification of such. Antibiotics from the fluoroquinolone subclass were studied and separated by reversed-phase HPLC on Discovery RP-AmideC16, Discovery C18, and Discovery C8 columns.

Gabapentin is an analogue of +/-aminobutyric acid (GABA), a substance approved by the US Food and Drug Administration (FDA) for add-on therapy in treating seizures thought to be associated with interference in GABA-ergic pathways or provoked by excitory amino acids. Gabapentin circulates essentially unbound to serum proteins. This application note describes the use of reversed phase HPLC in the quantitative analysis of Gabapentin extracted from serum.

Acrylamide is a commonly analyzed food-borne carcinogen. In this application note, Sigma-Aldrich present convenient kits, LC and GC columns and sample prep methods for fast and reliable analysis of acrylamide in food.