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Quantitative Analysis of Gabapentin from Serum by Reversed Phase HPLC using Discovery C18 Columns

Sigma-Aldrich Supelco
20 Jul 2015

Gabapentin is an analogue of +/-aminobutyric acid (GABA), a substance approved by the US Food and Drug Administration (FDA) for add-on therapy in treating seizures thought to be associated with interference in GABA-ergic pathways or provoked by excitory amino acids. Gabapentin circulates essentially unbound to serum proteins. This application note describes the use of reversed phase HPLC in the quantitative analysis of Gabapentin extracted from serum.

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Discovery C18 HPLC Columns

Sigma-Aldrich Supelco

Classic reversed-phases for all general-purpose applications. Features: Excellent peak shape and efficiency compared to competitive columns Classic reversed-phase retention and selectivity Excellent, no-bleed character for LC-MS applications C18 is generally more retentive than the C8 Column Details: Chemistry: C18 Particle Size: 5 µm Pore Size: 180 Å Length: 20, 30, 50, 100, 125, 150, 250 mm ID: 2.1, 3.0, 4.0, 4.6, 10.0, 21.2 mm

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Drug TestingIt is essential to test the efficacy and toxicity of drugs at every stage of their development to ensure that a pharmaceutical product is fit for purpose and safe for use before reaching the stage of mass production and distribution.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.PharmaceuticalsPharmaceuticals are medicinal drugs used in healthcare to diagnose, prevent, cure and treat illnesses. Pharmaceuticals that are excreted after use appear in wastewater and can have detrimental effects on the environment.Human SerumHuman serum is found in blood plasma. The protein is used in a variety of applications including tissue engineering, transplantation and cell therapy.Drug MetabolismDrug metabolism is the enzymatic conversion within the body of pharmaceutical substances into other chemical compounds. The primary site for drug metabolism is the liver.

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