Direct-zol™ RNA Miniprep Plus by Zymo Research

Manufacturer Zymo Research  |   Model: R2070
4.8
/
5.0
  |  246 reviews
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Spin column procedure bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.


Direct-zol™ RNA Miniprep Plus by Zymo Research product image
Direct-zol RNA Miniprep Plus Kit
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Average Rating: 4.8
246 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2022

  • Organization: ASU



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great yields, good value!
Rating: 4.3

  • Application Area: Analyzing metabolites in human cells

"This kit provides high yields for an affordable value. Moreover, what I like about all Zymo kits is that you can order just one of the components if it runs low, while in most others you have to buy a new kit!"

Review date: 20 May 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: univerisity



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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It is good
Rating: 3.7

  • Application Area:Tissue samples

"It's an easy of use product, good balance between value and money"

Review date: 31 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: UNAM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good results in short time
Rating: 5.0

  • Application Area:Analyze gene expression

"Easy to use, with great results."

Review date: 30 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: ESALQ/USP



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product!
Rating: 5.0

  • Application Area:RNA extraction from tissue

"The kit is easy to be used, the final product (RNA) quality was excelent."

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Northern Arizona University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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By far the easiest method I've used.
Rating: 4.3

  • Application Area:RNA isolation from embryos

"DirectZol is super easy to use and I routinely get excellent quality total RNA (e.g., RINs 9-10)."

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: National Institute on Aging, NIH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Highest quality reagent for RNA isolation
Rating: 5.0

  • Application Area:RNA isolation from small cell numbers

"Compared to other RNA isolation kits, the Direct-zol RNA Miniprep plus maximizes the amount of RNA isolated from a small sample size. The ability to go directly from TRI Reagent/trizol along with the on-column DNA digestion step reduces sample handling to minimize RNA loss. The RNA is of high quality allowing for several different downstream uses."

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: National Institute on Aging, NIH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Highest quality reagent for RNA isolation
Rating: 5.0

  • Application Area:RNA isolation from small cell number

"Compared to other RNA isolation kits, the Direct-zol RNA miniprep plus maximizes the amount of RNA isolated from a small sample size. The ability to go directly from TRI Reagent/trizol along with the on-column DNA digestion step reduces sample handling to minimize RNA loss. The RNA is of high quality allowing for several different downstream uses."

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: UConn Health



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent kit for excellent results
Rating: 5.0

  • Application Area:RNA extraction from cells and mouse tissues

"I love Zymo products and I cannot wait to open my own lab someday and use exclusively Zymo reagents. I have never had issues with their products and this RNA miniprep kit works wonders for Trizol reactions. It is very dynamic and have gotten me great yields for many papers so far."

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Delaware State University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Good results, compared with competitors.
Rating: 4.3

  • Application Area:Plant viruses

"This kit produces high quality RNA, which can be concentrated using a small volume of elution buffer; no need concentrating afterwards."

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Miami University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great cost effective and fast kit
Rating: 5.0

  • Application Area:Transcriptomics

"I used the Direct Zol RNA miniprep kit to isolate total RNA from my bacterial cultures. I was able to receive a high yield with no problem and the kit only takes 20 -30 minutes in total to finish up the whole process. We have purchased this kit multiple times and I highly recommend it as well."

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus


  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results! easy to use
Rating: 4.3

  • Application Area:Analyze gene expression in cell lines

"Great results! Easy to use, very reproducible"

Review date: 29 Mar 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: NIOF



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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I highly recommend it
Rating: 4.7

  • Application Area:Extracting DNA for PCR applications

"Easy to use, it gives high quality DNA yield, it can be used for PCR applications, it gives reliable results. Good efficiency in time and money saving."

Review date: 01 Feb 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCI-Medical Center



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Easy protocol and good yield results.
Rating: 4.3

  • Application Area:Extract RNA for further analysis from tissues and cells.

"It has better quality and yields RNA. The protocol is easy and simple and the result is consistent among samples."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: GWU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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It worked!
Rating: 5.0

  • Application Area:QRT PCR

"They came on time. Got good quality and yield of RNA. Would highly recommend."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Plant Sciences Inc.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great RNA extraction kit ever
Rating: 5.0

  • Application Area:RNA extraction from plant tissue

"I used Direct-zol RNA Miniprep Plus to extract RNA from plant samples. It ended up in high quality and quantity."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: UMW



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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This kit was comparable to others I've used
Rating: 4.3

  • Application Area:Analysis of brain tissue

"The kit worked well and was easy to use. I analyze brain tissue (hippocampus and amygdala) and needed to wash multiple times to eliminate fat. Otherwise was able to use standard protocol that came with kit."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Genome Explorations



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fantastic Products.
Rating: 5.0

  • Application Area:Extraction of RNA for Next Gen Sequincing

"Zymo Research products are by far the easiest kits to use and are extremely effective without and surprises."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Umass Amherst



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product. Time saving. Labor saving!
Rating: 5.0

  • Application Area:RNA extraction for gene expression

"I really appreciate your products which saved a lot of time for me. As a graduate student and a mother of two kids, time is everything. I love your products. By the way, separated purchase available is very consumer friendly. Like the choice a lot."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Narayan Lab @NIH/NIDDK



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Time-saving alterative to traditional RNA isolation.
Rating: 4.7

  • Application Area:RNA isolation from iPSC-derived brain cell types for for qPCR and RNA-seq analysis and validation

"The Zymo RNA MiniPlus kit is very user-friendly. It achieves RNA isolation through a column-based approach. The benefit is a time-saving prep that yields high-quality RNA as analysis by NanoDrop spectra. The one limitation is the column lowers the total RNA yield. Flushing of the column helps to recapture total RNA. This is a great alternative to traditional Trizol and phenol: chloroform isolation protocols which require special waste procedures for both phenol-containing liquid and plastic waste."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Altay Therapeutics Inc



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast workflow and good quality RNA! Recommend!
Rating: 4.7

  • Application Area:RNA extraction from cells

"This kit is quite easy to use. The RNA quality is very good. The workflow is very easy and quick. The only thing about it that is a little frustrating is that the tubes a slippery and not so easy to handle. They dont have good grip. You have to be very careful or they'll slip out of your fingers."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: CIRB - Collège de France



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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The kit was very easy to use, very fast and provided good results
Rating: 4.7

  • Application Area:Analyse gene expression in human (post-mortem) brain tissue

"Direct-zol RNA Miniprep Plus Kits are great! I'm very satisfied with the efficiency/quickness of RNA extraction from human brain samples, especially when we need high quality RNA for quantification and sequencing. This product is extremely easy to use and provided a good purity of the extracted RNA. And finally, the technical support were very helpful and very responsive."

Review date: 31 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: ASDRP



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome!
Rating: 5.0

  • Application Area:Extract bacterial RNA for analysis

"The instructions were easy to follow and I got a good and pure yield for the PCR experiment I needed to conduct using the RNA."

Review date: 27 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of Pittsburgh



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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The kit was very easy to use and provided good results.
Rating: 4.0

  • Application Area:Sequence RNA

"The TRI-reagent should always be handled inside of a fume-hood. If you are planning to work with more than 20 samples, a second kit is necessary to have enough of the flow through columns."

Review date: 26 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of Nevada, Las Vegas



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, will stick with Zymo for a while.
Rating: 5.0

  • Application Area:RNA isolation for PCR

"This kit is very easy to use and quick to use. I was able to achieve high yield with clean results time after time. I was able to use it for blood, cheek swabs, and saliva. Anytime I called support, they were very helpful and very responsive. I couldn't be happier."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: UCR



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, affordable, easy to use!
Rating: 5.0

  • Application Area:RNA extraction for insect samples

"I used this kit to extract RNA from insects and need to detect a RNA virus from it. The kit is very easy to use, save time, and all can be performed under room temperature. I can extract RNA from a singe insect as well as a pool of insects. The chemical reagents within the kit are super enough for extraction uses. This kit is cheaper than Qiagen kit, so it is more affordable. And the extraction result is also very good! BTW, the technical support is very helpful!"

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Georgetown University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good value for money.
Rating: 5.0

  • Application Area:RNA isolation

"Good value for money. Zymo doesn't rip you off, unlike other companies."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Houston Methodist Research Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit! Use it whenever possible.
Rating: 5.0

  • Application Area:Clean and prepare cytoplasmic RNA for capping analysis.

"This product is extremely easy to use. I love that all of the needed reagents come prepared and aliquoted for continued ease of use. The protocols are easy to follow and provide reproducible results."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Texas Tech University Health Sciences Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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User friendly!
Rating: 5.0

  • Application Area:Analyzing the oncotargets

"The product is quite user friendly. The results were reproducible and high quality. Everyone should know about the great Zymo support team who helps when needed as far as technicalities are concerned. It is a 5/5 for me in all aspects."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Houston Methodist Research Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great kit for beginners and very fast.
Rating: 4.7

  • Application Area:RNA Extraction

"The DirectZol kit is great (most of the time). The RNA yields from cell culture are reproducible and the purification process is a "piece of cake." Anyone with novel experience can use this kit and get great results FAST."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: UIC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good product and quality
Rating: 5.0

  • Application Area:Chicago

"If I place order the product today, then I can receive it the next day. After using it, the product is high quality and shows my result. Compare with other vendors, the price is a reasonable. Thank you."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of Minnesota



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The samples were clean and the concentrations were good
Rating: 5.0

  • Application Area:Preparing the samples for qPCR runs

"The samples were clean and the concentrations were good."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Yale School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results
Rating: 5.0

  • Application Area:RNA isolation from sorted cells

"I have used this kit for the isolation of RNA from a small number of sorted cells. It worked great, we sent it for RNA-seq and got incredibly good results."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Expert Reviewer

  • Member since: 2016

  • Organization: University of North Carolina at Charlotte



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great Results and Quantity Providing RNA purifying Kit
Rating: 4.3

  • Application Area:To Purify the RNA

"A great kit to increase the quantity and purity of the extracted RNA. I am very happy to use it for my lab samples which needs high quality RNA for quantification and sequencing. Very amazed with the productivity of this kit."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: university of vermont



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Works well to provide pure mRNA at a good price.
Rating: 5.0

  • Application Area:mRNA isolation from whole tissue in Trizol

"Our lab tried this product after years of Trizol/Chloroform extractions. We needed a kit that would produce *clean* mRNA with little Trizol carryover for downstream RNAseq applications. This kit did its job well and produced clean, pure mRNA and is less expensive than RNeasy and RNaqueous counterparts. The Zymo website also provides a brief video of the protocol which we found to be a helpful introduction. Our procedure, briefly: After grinding the tissue in liquid nitrogen and adding Trizol, we passed the mixture through a Qiashredder. After pelleting the debris, the Trizol/lysate supernatant was put through the Direct-Zol kit."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: USUHS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great quality results!
Rating: 5.0

  • Application Area:Analyze transcripts in a tissue sample

"It is easy to use. Great quality end product."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: university of florence, Italy



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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RNA extractions with optimal RIN score, NGS ready
Rating: 5.0

  • Application Area:NGS ready total RNA in minutes

"I had to extract 50 samples for total RNA, these samples had to be sequenced for splice variants. Considering that the number of samples were quite a few, I did opt for the traditional method nor the Qiagen (as the DNAse treatment is a column and not a physcial enzyme). I used the RNAzolkit, and within 15 mins is at least 95% of the samples with RIN of >7. This kit is rather easy to use with the DNAse I digestions step in the column itself, so that was good. The NGS run was great so this one is good especially because you can also store your RNA in TRIZOl and extract the RNA whenever you want without the fear of degradation."

Review date: 25 Jan 2022 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Universidad Juárez del Estado de Durango



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Excellent product I think is the best with regards to cost benefit relation
Rating: 4.7

  • Application Area:Gene expression in tissue samples

"It is really very easy to use, we just had the problem with the sample homogenization, we were working with pancreas tissue and the RNA was easily degraded, but this kit was the option with one we got the best result using a homogenization with trizol in low temperature."

Review date: 16 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Texas at San Antonio



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Easy to use
Rating: 3.3

  • Application Area:Analyze RNA

"Product was effective and easy to use. Price is also okay"

Review date: 16 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: UNAM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best tool for RNA isolation it helps to achieve high quality and purity
Rating: 5.0

  • Application Area:Analyze gene expression

"Direct Zol was the best tool for RNA isolation it helps to achieve high quality and purity from my samples also reduces the time consuming compared with the traditional method"

Review date: 16 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: University of Cordoba



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results. It´s my favourite method for RNA isolation
Rating: 5.0

  • Application Area:RNA isolation in tissue samples for gene expression analysis

"An excellent method to quickly isolate high-quality RNA directly from samples in Trizol, TRI Reagent or similar from different sample sources (cells, tissues, serum, plasma, etc.)."

Review date: 16 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Texas A&M University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fantastic kit! I look forward to using it again.
Rating: 5.0

  • Application Area:Isolation of insect RNA for RNA sequencing and de novo transcriptome assembly

"I used Zymo's Direct-zol RNA miniprep kit for the isolation of RNAs from specific caterpillar tissues for Next Gen Sequencing. Because I was isolating RNAs from multiple different tissues of each individual, I had to process 100s of samples! I was able to rapidly isolate high-quality RNA from the tissues and obtain high yields. This kit really saved the day when it came to processing 100s of tissue without losing any quality of isolated RNA in the sample!"

Review date: 16 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: ASDRP



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Extremely good at extracting RNA, would definitely recommend!
Rating: 4.7

  • Application Area:RTqPCR/RNA extraction from cancer cells

"The Miniprep Plus was extremely good at extracting RNA in sufficient concentrations. The instructions were also very clear, but I think it would have been beneficial to have more volume of each solution. Other than that, it was great!"

Review date: 16 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: University of Ilorin, Ilorin



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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The RNA yield and purity was simply fantastic
Rating: 4.3

  • Application Area:Extraction of RNA for gene expressions studies

"The RNA yield obtained using the kit was great and the purity was ok to perform any throughput analysis."

Review date: 16 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Sao Paulo



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great result and easy to use.
Rating: 5.0

  • Application Area:RNA sequencing

"I achieve high quality RNA that is used as an input for RNA sequencing."

Review date: 15 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UC Berkeley



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Efficient RNA extraction in 0.5 h and good quantity/quality of RNA from small tissue.
Rating: 5.0

  • Application Area:Extract RNA from small tissue like choroid plexus to explore differential gene expression

"The sample kit is a good start for my first trial of RNA prep and it is very useful for me to compare different kit based on my sample size."

Review date: 15 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Florida



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results and very easy to do the assays
Rating: 5.0

  • Application Area:Analysis of mRNA level changes

"The kit is very easy to use, even my undergraduate students can finish the experiments very well and fast, the RNA quality and yield is pretty high. The price of this kit is reasonable."

Review date: 15 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Virginia Tech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great RNA quality.
Rating: 5.0

  • Application Area:RNA extraction from mosquito midguts

"The product is very easy to use, in a relatively less amount of time with better RNA quality."

Review date: 15 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: NYU Grossman medical school



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent value for money
Rating: 5.0

  • Application Area:RNA isolation from primary cells.

"The protocol is straightforward and easy to use. Compared to red/blue it's also relatively cost effective. The yeild concentrations are spectacular!"

Review date: 15 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UNC Charlotte



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great Results, with High Efficiency
Rating: 4.0

  • Application Area:Good Kit to Work With

"I like the capacity of the kit. It provides good results and very easy to use. Highly efficient and cost effective. Very happy to have and would recommend for the best RNA procedure."

Review date: 15 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Uni of Hawaii-JABSOM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 5.0

  • Application Area:RNA seq

"This item is very useful for the quick extraction of tissue RNA samples with high purity."

Review date: 15 Dec 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: MGH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, easy to use, worked well.
Rating: 5.0

  • Application Area:Extracting RNA from cells in culture

"Anyone who has tried RNA extraction from tissue/cell lines can attest to the frustrations that may be present throughout the process. From low yield to degradation, RNA extraction can be very challenging using any method. This kit was surprisingly easy to use, and gave great results. Will definitely be purchasing the full kit for future use."

Review date: 17 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: ASDRP



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great results! Would definitely recommend!
Rating: 4.3

  • Application Area:Extracting RNA from tissue culture

"This prep kit is easy to use and has all the materials. We were able to isolate the RNA in good concentrations, which we then used for our projects."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UC Berkeley



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Low yield from biofilmy bacterium, good yield from green alga..
Rating: 4.7

  • Application Area:Transcriptomics

"Unfortunately, this kit did not allow me to purify sufficient RNA from a tough-to-lyse bacterium that makes some unknown biofilmy stuff. However, it did result in high yield RNA from the alga chlamydomonas reinhardtii, albeit the quality assessed via bioanalyzer trace was just OK (not perfect)."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: ISU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results generated for NGS.
Rating: 5.0

  • Application Area:RNAseq

"Great kit. Easy to use and reliable results. Perfect for NGS applications."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Louisville



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results!
Rating: 5.0

  • Application Area:RNA extraction and gene expression

"Very easy to extract RNA, simple fast and good concentrations."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: SDSMT



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results. We use this kit very often in our lab now
Rating: 4.3

  • Application Area:RNA extraction from thermophiles

"The Direct-zol kit is very useful for extracting DNA from bacteria, and it worked just so well for our thermophilic cultures. The results and yields were reproducible too, and the transcriptomic data that was processed using the RNA extracted from RNA zol was of high quality."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: mayo clinic



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product and guarantee to get what you want.
Rating: 5.0

  • Application Area:Gene expression

"Using this kit to obtain RNA is easy and very effective. You can get higher yield and purer RNA. The RNA obtained is great for downstream application, such as RT-PCR, RNA sequencing, and gene cloning. Customer service is great too, very informative and helpful."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Kansas State Unvieristy



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product for RNA extraction.
Rating: 5.0

  • Application Area:RNA extraction for wheat plants

"Rapid and reliable isolation of RNA for qRT-PCR analysis in plants."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Perelman School of Medicine at the University of Pennsylvania



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Works with frozen samples
Rating: 5.0

  • Application Area:Neuroscience

"We had existing samples that were frozen in tri-reagent and these kits worked well for extracting high quality RNA."

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: GMU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great result.
Rating: 5.0

  • Application Area:Purify RNA from suspension cells

"This product is very easy to use. The protocol is simple to follow. I got great RNA quality and quantity. The qRT-PCR Results from the RNA were very clean"

Review date: 16 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: University of Penn



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent results.
Rating: 5.0

  • Application Area:RNA Purification

"Excellent to purify RNA in an easy and fast way with high quality and quantity RNA."

Review date: 15 Nov 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: The Ohio State University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Excellent products for consistent results!
Rating: 4.3

  • Application Area:Analysing recombinant RNA molecules

"The kit has been working very well with consistent results with good RNA quality and yield. The spun columns are handy compared to the Trizol method. The procedure is straightforward to follow as well."

Review date: 07 Sep 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Instituto de Investigação e Inovação em Saúde (i3S)



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great. Easy and efficient kit.
Rating: 5.0

  • Application Area:Adipose tissue

"Very interesting, feasible, and highly efficient method for RNA extraction from adipose tissue."

Review date: 21 Aug 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Virginia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Quick and easy isolation of high quality RNA.
Rating: 5.0

  • Application Area:RNA isolation from murine cell lines and tissues for RT-qPCR and RNA-seq

"RNA quality is very good and the kit is very easy to use, which makes the whole procedure more reproducible even in unexperienced hands. You can quickly get high quality RNA without worries of losing your RNA pellet."

Review date: 01 Aug 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: Marine Biological Laboratory



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Simple and robust kit with great price.
Rating: 4.3

  • Application Area:Transcriptomics

"Direct Zol is a simple and robust kit to get your RNA out of cells. You may better off with your turboDNAse treatment, althugh kit has its own DNAse in it and recomended to use following the protocol."

Review date: 29 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Minnesota



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results.
Rating: 4.0

  • Application Area:Gene expression

"This kit is easy to use and we can get enough amount of RNA for RT-qPCR from mouse skeletal muscle tissues and cultured cells. The protocol is simple and we do not need any special equipment for this kit. The price is not as expensive as other similar kits."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: ASDRP



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very easy to use and worth the money!
Rating: 5.0

  • Application Area:Extracting RNA

"This kit was very useful to extract RNA from cancer cells to use for qPCR experiments. The instructions were easy and efficient!"

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Suretox lab



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great yields, minimal time and authenticated downstream application results.
Rating: 5.0

  • Application Area:Using for TaqMan Assays for cytokines

"I have used so far 10 Direct-zol RNA Miniprep Kits to purify RNA from adherent and suspension cell samples lysed in Trizol and always performed the optional on-column DNAse digestion. The yields were extremely good, most samples show RNA yield more than expected over OD 260/280 2.0. I stopped using big brand kits, which take a much longer time to purify with lower yields and this Direct-zol RNA Miniprep Kit seemed like such a convenient alternative."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: ASDRP



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Amazing results for research work.
Rating: 5.0

  • Application Area:Analyze various biological samples

"It is easy to use, reliable, and accurate. Good for beginners as well."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Expert Reviewer

  • Member since: 2020

  • Organization: Colorado State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good efficiency and plasmid recovery.
Rating: 5.0

  • Application Area:Gene cloning

"Easy to use, reproducible results, and no special aftercare needed."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Washington University Biology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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DirectZol is wonderful for RNA isolation and transcription reaction cleanup.
Rating: 5.0

  • Application Area:Direct isolation of RNA from Trizol lysates; isolation of capped mRNA

"I find the Direct Zol RNA isolation kit the fastest, easiest, and highest yielding method for isolation of RNA from Trizol extracted Drosophila tissue lysates. So much easier than centrifugation followed by phase separation. I've also started using the kit to clean up capped mRNA T7 ARCA transcription reactions, and the yields are wonderful! Usually get 600 to 700 ng/microliter of clean useable mRNA for insect transgene injections. I highly recommend this product."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: UNAM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 5.0

  • Application Area:Analyze gene expression

"Is really easy to use and reduces steps on the traditional method to obtain pure RNA."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Northwestern University-Feinberg School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy and simple, High yield and high quality of RNA prep kit.
Rating: 5.0

  • Application Area:Total RNA extraction from live cells after FACS sorting

"We’ve been working to generate pipeline with bulk RNA seq. Our lab tried several kits from different companies, then decided to purchase the entire kit from Zymo after trying a sample kit. This kit gave us highest amount of RNAs with hight quality for RNA-seq from both whole tissue and cells collected from FACS. The columns works perfect with Trizol agent-which is the most common reagent and in-column digestion to remove DNAs was another benefit! Easy to follow the directions and reasonable price for 50 preps, compared to other competitors. Highly recommend."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: The Jackson Laboratory-GM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Does the job with ease.
Rating: 5.0

  • Application Area:RNA-seq, quant-seq, qPCR

"Direct-zol™ RNA Miniprep Plus is excellent value for money over similar products."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: Universidad de Córdoba



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use. Good yield.
Rating: 4.7

  • Application Area:Isolation of nucleic acids

"It was easy to manipulate, product isolated was good on both yield and quality."

Review date: 28 Jul 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: DermBiont



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Good performance.
Rating: 4.3

  • Application Area:RNA extraction for RNAseq

"The RNA yield was better than other products, but the column-based method does not have a high throughput. It would be helpful if the product can be improved for automated pipelines."

Review date: 09 Jun 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Witwatersrand



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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RNA extraction has never been easier.
Rating: 4.7

  • Application Area:RNA extraction

"Gives you great quality RNA in high concentrations every time! It’s very simple and easy to use and instructions are crystal clear. "

Review date: 07 Jun 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Toronto



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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It is good.
Rating: 4.0

  • Application Area:Nucleic acid - protein extraction

"It is easy to use, you can use trial in the beginning. The protocol is short and effective. Technical support is good."

Review date: 26 May 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: UNMC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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New staple in our lab!
Rating: 5.0

  • Application Area:RNA Isolation

"This product is quick and easy to use. Very reliable with clean RNA. We switched from a standard RNA isolation method to this product because of its consistency and reliability of the final product."

Review date: 14 May 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Texas A&M University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome kit, would recommend to other scientists.
Rating: 5.0

  • Application Area:RNAseq

"This is a great and easy kit that reliably gives really high quality and great yield as well. Our lab's go-to for RNAseq projects."

Review date: 18 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Assam University, Silchar, Assam, India



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Fantastic package! Simple to use and extremely dependable in terms of performance.
Rating: 4.3

  • Application Area:Pathway analysis in Yeast Cells

"This is a very simple and fast product for RNA extraction from a variety of yeast cells. Postgraduate and PhD students routinely isolate mRNA from several yeast species. We tested several species from Pichia, Candida, Saccharomyces, and Komagataella. On average we get RIN value of 9 for the isolated RNA which is great for our downstream applications expression analysis. The recovery is good too, with yield ranging from 850-1000 ng/ul RNA. We simply love the on column DNAase digestion, clean up in 45 minutes. Above all, the price per sample fits to our budget. With wide range of distributors we get this product in the remote area in weeks. It fits the bill perfectly."

Review date: 18 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: UTMB



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good results.
Rating: 5.0

  • Application Area:PCR applications

"Efficient, high quality easy to use."

Review date: 17 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Berlin Institute for Medical Systems Biology at MDC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy, fast & reliable.
Rating: 5.0

  • Application Area:RNA extraction from human cell lines

"The product is extremely easy to use and saves time compared to the competition, without compromising the yield and quality of the extracted RNA."

Review date: 17 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UC Berkeley



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome results, very happy with the product!
Rating: 5.0

  • Application Area:RNA isolation

"I have been using this product for a year now and it works very good for RNA isolation from all kinds of samples."

Review date: 17 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: SUNY BINGHAMTON UNIVERSITY



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Student friendly.
Rating: 5.0

  • Application Area:Drosophila studies

"Absolutely easy to use. The students were able to complete the experiment independently and the results were very similar to the results from Qiagen."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Expert Reviewer

  • Member since: 2020

  • Organization: Colorado State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Easy to use, good results.
Rating: 5.0

  • Application Area:Molecular cloning

"High quality and clean recovery of products, Easy to use, Fast and time saving."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Massachusetts General Hospital/Harvard Medical School



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results. Cannot go back to other similar products!
Rating: 5.0

  • Application Area:Miniprep for vector cloning

"It is easy to use, I especially love that we do not have to pellet the bacteria and resuspend before lysis. The yield and quality is good as well. Worth the money, and you save time and work."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Illinois



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results!
Rating: 5.0

  • Application Area:Analyze mRNA in bovine tissue samples (liver, uterus, uterine smear, rumen pappillae...)

"I found this kit one of the easiest ones to extract RNA from tissue samples and it works very nicely as well with smear samples (from uterine content). The protocol steps are easy to follow and I have always received the product in great condition - I bought it several times already!"

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UC Berkeley



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Greatest.
Rating: 5.0

  • Application Area:Isolate RNA from cells and tissues

"Great product."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Pacific University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Best for the price, but get other DNase.
Rating: 4.3

  • Application Area:RNA expression tracking

"Overall, I think this is a great product. It is easy to use and the steps are pretty clear. You can't beat the price either. My only negative comment is that the DNase doesn't seem to work very well. Our labs ended up buying it from another supplier and just using it with this kit."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Southern California



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Quick isolation of RNA for sequencing or PCR.
Rating: 4.3

  • Application Area:RNA isolation for sequencing

"The product comes easy to use, with minimal steps after trizol application to 2D tissue culture on plastic or glass. The purity comes out pretty close to what is reported, although it seems less consistent when cell counts are lower. Time of isolation is cut significantly. I think it is worth it if you are willing to pay a premium compared to traditional methods of isolation."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UNAM



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Compared to other kits it is very good.
Rating: 4.3

  • Application Area:Lymphocyte mRNA extraction

"The procedure is clear, easy to carry out, the amount of mRNA was lower than expected and the quality was regular, we are in the standardization phase, I hope that once the points to be corrected have been identified, the performance of the insert can be achieved."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Universidad Autónoma de Yucatán



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent result and easy to use.
Rating: 5.0

  • Application Area:Extraction DNA and RNA

"We purchace Zymo Resesrch because, the data we get is better in comparison with other companies."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Princeton University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good product and saves your time.
Rating: 5.0

  • Application Area:RNA extraction

"This product is really easy to use. Take less time and generate high quality RNA in most of my samples."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Clemson University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Amazing results. Easy to use.
Rating: 5.0

  • Application Area:Extracting RNA from cell culture samples

"This product is 100% effective when used in extracting RNA from cell / tissue samples. The simplicity of the protocol is amazing. It is well detailed and easy to follow. The RNA yield is usually pretty accurate based on my hypothesis. Also, the product content last a long time."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: North dakota state University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results from this kit.
Rating: 5.0

  • Application Area:RNA isolation from cells and tissue

"This kit gives reproducible results all the time I use it and the most important thing is that it is very easy to use just by following the procedure."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Kansas State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Time efficient, great reproducibility.
Rating: 5.0

  • Application Area:Virus detection and human immunology

"I have used exclusively Zymo products for 10 years now in all my RNA and DNA extractions. Great products, increase reproducibility, easy to follow protocols."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: CSS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Takes the worry out of isolating RNA.
Rating: 5.0

  • Application Area:Cleaning and isolating RNA from a variety of samples

"This is my go-to product for the application. It's easy to use, quick and efficient. The protocol is clear enough that students can handle the extraction process too."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Mount Sinai



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The Zymo kits are easy to use and cheap! The results are awesome.
Rating: 5.0

  • Application Area:Molecular biology - extraction of RNA

"The product is cheap and easygoing. The results are delightful."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Tulane National Primate Research Center



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great kit for isolation of good quality RNA.
Rating: 4.7

  • Application Area:Analyze RNA in samples

"Good quality RNA can be obtained with easy to use kit."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Clemson University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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High purity, and easy protocol.
Rating: 5.0

  • Application Area:Purify the total RNA from eukaryotic cells

"The protocol is easy to use and the produced total RNA is high purity and does not contain genomic DNA, since there is a step for DNase incubation during purification. The yield is comparable to other vendors."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Brown University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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High quantity with good quality of RNA sample.
Rating: 4.7

  • Application Area:RNA isolation from tissue samples

"It worked nicely. I got a lot of RNA with good quality. 260/280 is 2.5."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: COLUMBIA UNIV



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product, highly recommend.
Rating: 5.0

  • Application Area:Extract RNA from tissue sample

"A very good product, has detailed protocol, easy to use and can get good results. Highly recommend."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Yale School of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 5.0

  • Application Area:RNA isolation from PBMC

"The product was good and RNA quality was good."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Virginia Tech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Definitely great results obtained really easily.
Rating: 4.7

  • Application Area:Digestion of mosquito midguts for RNA

"The RNA yields were pretty good, it was easy to use and definitely worth the money."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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I obtained reproducible results and it is easy to use.
Rating: 4.0

  • Application Area:Pasmid isolation for KO clones

"I obtained reproducible results and it is easy to use."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Cornell University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, good yield!
Rating: 5.0

  • Application Area:RNA seq experiments from samples

"Direct-zol RNA Miniprep Plus kit is the best in the market for RNA extraction. It is easy to use and gives great yield. The best part is that it can also extract small RNAs from samples. I would definitely recommend for use to everyone!"

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: UMASS Medical School



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Strongly recommended to isolate RNA from mycobacteria and eukaryotic cells.
Rating: 5.0

  • Application Area:RNA isolation

"Reliable and consistent quality of RNA obtained when using these Zymo kits."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Boston children’s hospital



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Reliable product and wonderful service.
Rating: 5.0

  • Application Area:RNA extraction and qPCR

"It’s a very good and reliable product. We use it to extract RNA from fungal samples, and it’s quite easy to use and compatible with trizol stored samples. The only thing is that we tend to have to do a second round of DNAse digestion to remove trace amount of gDNA contamination. The customer service is very nice and once they even offered to fill up our back ordered kit with free samples (didn’t go for that but really appreciate their kind gesture to do anything possible to care for the customers). RNA quality off the column is always good."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Institut Pasteur Montevideo



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great and esay to use extraction and purification RNA kit.
Rating: 5.0

  • Application Area:Molecular biology

"Really ease to use RNA purification kit directly from phenol/trizol or directly extraction from cell. I use in general from trizol (cells ans tissue). It work really nice, with very good RIN number (>9 in general) generated in bioanlayzer equipment. RNA was used to downstream RNAseq analysis, cDNA generation an RT-qPCR without problems."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Nord University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Perfect RNA extraction kit for the scientist who doesn't like to waste her/his time.
Rating: 5.0

  • Application Area:Total RNA extraction from the different types of animal tissues such as gills, liver, and embryos

"Direct-zol™ RNA Miniprep Plus is a very simple and fast resolution of the RNA extraction issues. RIN values usually higher than 9.0 for the fresh tissues."

Review date: 16 Mar 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Louisville



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product can’t live without it!
Rating: 5.0

  • Application Area:RNA purification

"Zymo's Direct-zol RNA Miniprep Plus is the bomb! It provides the easiest, best, and most economical way for us to isolate RNA from microbial cells (especially fungi). The on-the-column DNase is quicker and easier to use than other kits where the treatment has to be done separately."

Review date: 02 Feb 2021 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: DEPARTMENT OF GENETICS UNIVERSITY OF DELHI SOUTH CAMPUS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results obtained.
Rating: 5.0

  • Application Area:RNA isolation from animal cells

"Used this product for RNA isolation from animal cells. The quality of isolated RNA was very good in terms of RIN value. Used the isolated RNA for cDNA synthesis, gene-expression microarray and transcriptome sequencing."

Review date: 09 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: nyu



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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This is an easy to use RNA prep kit with good quality.
Rating: 5.0

  • Application Area:System biology

"This is an easy to use RNA prep kit with good quality."

Review date: 08 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2004

  • Organization: College student



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results with great price for the kit.
Rating: 5.0

  • Application Area:RNA extraction for RNA sequencing

"Easy protocol, got desirable RNA quality."

Review date: 05 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Boston Children’s Hospital



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very reliable product and easy to use.
Rating: 5.0

  • Application Area:Define expression level in Candida Albicans

"This is a very good product that was recommended by my colleagues. It is suitable for the extraction of yeast/Candida total RNA from samples stored in Trizol. It is very easy to use, takes around 30-40 total to finish the extraction, on column DNase digestion and the final concentration reading. The recovery is good too, I ususally get 800-1000 ng/ul RNA from the column. One thing to notice that is these columns do have maximum capacity. Since we tend to have slightly higher cell numbers to apply to the column, we have used an additional ‘in the tube’ DNase digestion step to make sure the complete removal of gDNA contamination. We also purchase the columns separately just for regular RNA cleaning procedure. All the qRT results look quite good."

Review date: 05 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: DUKE



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Amazing kit! Easy to use and very reliable to obtain results. Highly recommend it!
Rating: 5.0

  • Application Area:RNA extraction in PBMC and human tissue

"The quality I am looking for in PBMC is usually as high as possible in both ratios 260/280 and 260/230 because these samples are usually sent for sequencing and many of them are nor replaceable. This kit, in addition to be easy to use and to explain to others, ensure high yields and ratios if it is used properly. Highly recommended to use!"

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UCLA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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I like this product so much, saving me from RNA extraction.
Rating: 5.0

  • Application Area:RNA extraction from tissue samples

"1. It's easier to use with column filters than regular Trizol extraction of RNA. DNase I included guarantees the quality of RNA without DNA contamination, good for various downstream applications. Also, the small RNA is collected and can be used for more comprehensive RNA profiling. 2. I remember once I contacted the technical support for some questions, I got the reply pretty quick. That makes me more confident in the company's devotion on customers. 3.The price is more competitive compared to other companies, considering the performance and easiness."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: Keck of USC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Save time! Save money! Save life!
Rating: 5.0

  • Application Area:Transcriptom in human samples

"The Direct-zol RNA Miniprep Plus Kit is very effective for purifying total RNA from Human cancer cells. The protocol is pretty straightforward and time efficient. The RNA quality is very high. I used the RNA for the total RNA sequencing. The data is great with average read quality higher than 35.50. And the results are very reproducible. I strongly recommend this product."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Colorado State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, easy to use, great quality reagents.
Rating: 5.0

  • Application Area:Plant tissue for viral testing

"This product yields amazing results with easy to use instructions. It is a time saver - a big plus for the lab. Very cost effective and highly reproducible. High quality RNA. In my lab, students can follow instructions without having prior experience minimizing errors and repeats. Quality of reagents is superb. I would highly recommended."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UNC Chapel Hill



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results in less time than using conventional methods.
Rating: 5.0

  • Application Area:RNA isolation

"This kit significantly reduces the time of extraction without compromizing the quality of RNA. The protocol is very easy to follow and it does not require any special reagent preparation. I strongly recommend it for tissue and cells."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: JIPMER



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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It's good to use.
Rating: 5.0

  • Application Area:RNA extraction

"Its easy to use and provides good quality RNA."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UCR



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Good product.
Rating: 4.7

  • Application Area:Analyze gene expression in plant leaves

"It is easy to use and saves a lot of time. It also has a lot of DNA contamination. I am not sure if other users have the same problems."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: USC



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Easy to use.
Rating: 4.7

  • Application Area:RNA extraction

"Easy to use and reproducible if you follow all the steps of their protocol correctly!"

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UTMB



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great recovery rate of viral RNA from tissues and cell cultures.
Rating: 4.3

  • Application Area:Virus genome detection

"This kit is more affordable and processes samples faster than those from competitors. Recovery rate of RNA of interest is higher than 90% In our hands. This kit is what we use for all our viral RNA extraction work."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UAB



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Great readouts, helped streamline the workflow.
Rating: 3.3

  • Application Area:Cancer research, skin care

"Easy to use, very user-friendly process."

Review date: 04 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Pennsylvania School of Engineering



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product for viral RNA extraction.
Rating: 5.0

  • Application Area:Viral RNA isolation

"It is a really nice product. I was able to isolate viral RNA from the HEK Transfected cells. HEK cells were transfected with pnL4-3 to produce virions."

Review date: 03 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UMaine



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    5 out of 5
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Great kit. Pure products with easy to modify, as needed, instructions.
Rating: 4.0

  • Application Area:Isolation of total RNA from tissue (zebrafish)

"The kit is useful when obtaining high quality RNA, but does require longer time usage of DNaseI than mentioned. Overall, it is an efficient kit that yields good product. Additionally, the instructions are clear and straight forward and easy to modify is needed."

Review date: 03 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: URMC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, easy to use!
Rating: 5.0

  • Application Area:Extract RNA from primary CVL samples

"This kit worked great for what I needed! I needed to extract RNA from CVL samples for metatranscriptomics and it was perfect for the job, very easy to use."

Review date: 03 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Cornell U.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Works great!
Rating: 5.0

  • Application Area:RNA extraction from E. coli for gene expression measurements.

"Works well, is fast and easy. No complaints. Yield is sufficiently high for our purposes."

Review date: 03 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: university of florence, Italy



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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NGS quality RNA without losing precious time.
Rating: 5.0

  • Application Area:RNA within minutes, NGS quality

"I had tons of RNA in trizol and was looking for a spin column alternative instead of the usual precipitation. I came across this kit and order a sample kit. This is simple to use without the fear of RNASes. Plus the DNASe is provided as well which is a good touch. I also ended up doing a bioanalyziner and the RIN was always been 8-9 which was brilliant. (Plus one sample was in trizol for more than a year and the RIN was 9). I did some NGS with these samples and was satisfied. Can't complain, thumbs up!"

Review date: 03 Dec 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: ISU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results using this kit
Rating: 5.0

  • Application Area:Next generation sequencing

"Easy to use, good yield and great kit for degraded samples."

Review date: 10 Nov 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Oklahoma State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit that saves your headache for something else!
Rating: 5.0

  • Application Area:RNA isolation for RT-qPCR of porcine tissues

"I use this kit to extract RNA from the duodenal and liver tissue of pigs. The protocol is easy to follow and the results are very satisfying. A step by step guide from the manual will help you through and it does not take much time. Good value for money."

Review date: 20 Jul 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Rhode Island



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Works well and always gave good quality RNA.
Rating: 5.0

  • Application Area:Extract RNA from tissues that are hard to extract from

"I used this kit with a modified method, still doing phase separation with tr-regent and then putting the aqueous layer through the column in this kit. That worked really well for me and gave consistently good RNA. This modified protocol worked for polysaccaride rich tissues - mytlus mussel species that needed some extra care to extract good RNA."

Review date: 17 Jul 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Rhodes University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Obtained great results relative to equivalent kits from alternative vendors.
Rating: 4.7

  • Application Area:RNA isolation for qPCR

"The kit combines ease of use and speed together with the ability to isolate high concentrations of high quality RNA, with little to no contaminating DNA that could interfere with downstream applications such as RT-PCR and microarray analysis. It worked well for both Gram-negative bacteria (E. coli and S. typhimurium) and hard to lyse mycobacterial species, such as M. tuberculosis."

Review date: 17 Jul 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: University of Southampton



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Super easy to use.
Rating: 5.0

  • Application Area:Plant RNA extraction

"Super easy. I haven't benchmarked it against other kits, but happy with the output."

Review date: 17 Jul 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: Universidad de Córdoba



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great, fast and good quantity and quality of isolated nucleic acid.
Rating: 4.7

  • Application Area:Isolate metabolites in plants

"Great, fast and similar quantity and quality of isolated nucleic acid, good performance in cDNA synthesis."

Review date: 17 Jul 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Washington



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results and good yield.
Rating: 5.0

  • Application Area:RNA extraction from Drosophila tissues

"Easy to use. High-quality results and reproducible. Will recommend this product."

Review date: 06 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Oregon Health and Science University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great support.
Rating: 4.7

  • Application Area:RNA extraction

"The Zymo support team was very helpful in assisting us to find the right product for our needs. They gave us free samples, and checked in to make sure they were the right fit for our use."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: nyu



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use product and gives good quality results.
Rating: 5.0

  • Application Area:RNA sequencing

"This product is time saving and easy to use. It gives good purity and all kinds of RNA, which is good for RNA research."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UCR



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use, good quality RNA.
Rating: 5.0

  • Application Area:Reverse transcription qPCR

"This kit is easy to use and results in high quality RNA. We get consistent results using it for downstream RT-qPCR. We also have achieved good results with RNA-seq."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Cincinnati



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit, but not great with fatty tissues.
Rating: 4.7

  • Application Area:Analyzing RNA from cells or tissue

"This kit is amazing, but it has one technical issue. I used it for placental samples dissolved in RNAzol. The high fat of the samples make the column completely hydrophobic so water would pass after a spin down, but no RNA would be eluted. Outside of this instance, I've never had an issue."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of South Carolina Aiken



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Will be the go-to for my lab for RNA extraction for future.
Rating: 5.0

  • Application Area:Lysis of zebrafish tissue samples

"Product is extremely easy to use for degradation-sensitive material, is much less risky for exposure and contamination of phenol-related waste compared to traditional RNA extraction methods. Easy for undergraduates to use. High yield of RNA, high quality and works well for RT-qPCR directly."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Arkansas at Pine Bluff



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Good results.
Rating: 4.0

  • Application Area:Plasmid isolation

"I got good quality results by using this kit. It was excellent for restriction digestion."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: COLUMBIA UNIV



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very good product.
Rating: 5.0

  • Application Area:Tissue sample

"Easy to use, high production, a good product worth buying."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: NATIONAL INSTITUTE OF BIOLOGY



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Easy to use and efficient.
Rating: 4.7

  • Application Area:Transcriptomics of plant and insect tissues

"The kit is easy to use, the extracted RNA is of high quantity and quality. It's especially useful that Zymo provides kit versions with columns that have different RNA capacities and enable also smaller elution volumes."

Review date: 05 Jun 2020 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: McGill University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent product, highly recommended!
Rating: 5.0

  • Application Area:Transcriptomic analyses

"I used this product to extract RNA for transcriptomics with two bacterial species. It was an excellent product! It was easy to use, the protocol was straightforward and clear, and we got excellent quality RNA! It fared better than similar products that we have tried in the past. We were very impressed with the product!"

Review date: 13 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Marine Biological Laboratory



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Works fine but might require an additional RNA purification.
Rating: 4.7

  • Application Area:RNA extraction

"This kit is really easy to use. It's also quite fast! I like that it allows you to use samples already prepared in TRIzol. So I can homogenize my samples in TRIzol and store them at -80C until I am ready to proceed with RNA extraction. The downside is that in like 50% of time (10 out of 20 samples) I got a very bad 260/230 ratio (0.94-1.5) so I had to do additional RNA purification step. In short, I would say, yes, I recommend it if you are ready to perform an additional RNA precipitation or column clean-up step."

Review date: 12 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Louisiana Monroe



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results
Rating: 5.0

  • Application Area:Analysis of gene expression in cells

"Directzol provides high amount of RNA within a short time and the quality is good and reproducible."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Ribo-therapeutics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great reproducible research.
Rating: 5.0

  • Application Area:Analyze and quantify RNA

"I love Zymo RNA purification kits because they are easy to use and give reproducible data every time I use them."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Utah



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results. Reliable kit to use for molecular biology research.
Rating: 5.0

  • Application Area:Analyze the gene expression files of the tissue samples

"Very easy to use and the results are reproducible and stable."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UMass Amherst



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results and clean RNA!
Rating: 5.0

  • Application Area:Isolate RNA for the use in real-time PCR

"The RNA prep kit is excellent for yielding clean RNA in a user-friendly way. The steps are simple and easy to follow and were easily adapted with the RNA isolation protocol that was already in place. This kit is also great for RNA isolation from tissues; once the tissue is homogenized in the Trizol reagent, it can be spun to separate the aqueous phase before just following the rest of the Zymo protocol. The RNA is very clean every time and I am able to use a lower concentration of RNA for each PCR reaction!"

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2017

  • Organization: University of Delaware



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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High-quality RNA, just fine for NGS.
Rating: 4.0

  • Application Area:RNA extraction for NGS

"The RNA Miniprep delivers high quality and reproducible total RNA, which is ideal for short-read NGS applications. The recovery was high and the protocol was simple, rapid, and easy to follow"

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Texas Health



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Buy this and you'll thank yourself later!
Rating: 5.0

  • Application Area:Analyzing expression level of mutant proteins in cancer cell line.

"Direct-zol was a windfall for my research project! After spending months generating stable mutants, I then had to analyze target protein levels with and without chemokine treatment. As my P.I. usually prefers the "old-world" method and anything found Maniatis et al, I was originally directed to use a more common tri-reagent. However, the results were not reproducible. After spending weeks convincing the P.I. to get the Direct-zol kit, they relented, and it was so worth it. It is so much easier than handling large lysates, and reproducible every 3 times! The after sales care is pretty simple, just follow the reagent storage conditions. The bang for your buck is stellar because you get the answer you need and CAN MOVE ON! The only thing I can mention is that it may be worth eluting in two steps (no additional volume needed, just use 1/2 volume RNAse free water and soak column for 1 minute before spin-down, then repeat allowing 1-minute soak.)"

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Cincinnati



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Great results for cell culture, not so good for human placental samples
Rating: 4.3

  • Application Area:Placental samples

"This kit works amazingly with cell culture samples; however, RNA extracted from placental samples did not work. I think it was the high fat content. This made it so the elution buffer or water would not absorb into the column at the final stage. The water simply beaded on top of the column and still passed through upon centrifugation, but no RNA was eluted. This has only happened for placental samples in my hands and forces me to use phase separation. There was no indication this could happen in the manual."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: NYU



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results - recommended!
Rating: 4.0

  • Application Area:RNA extraction for quantitative PCR reaction

"Easy kit to use, detailed information, and great results. Recommended."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: NCSU



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very easy to use, quick and efficient RNA extraction
Rating: 4.7

  • Application Area:RNA extraction to look at gene expression of plants under stress, specifically anthocyanin pathway g

"High quality RNA was extracted using the Zymo kit. It was very easy, toxic free, and efficient."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Maine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Got good results from it!
Rating: 5.0

  • Application Area:RNA-sequencing

"I used this product during grad school and it works well. I also liked the service and the value we got for the product. I would order and use this again and I will recommend it to colleagues."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results
Rating: 5.0

  • Application Area:Stem cells

"I like this kit, it is easy to use and very fast."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Hope College Biology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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This is a great kit.
Rating: 5.0

  • Application Area:Isolate RNA for qPCR

"Very easy to use and gives great results. Make sure you use really good sterile water in your sample prep for best quality RNA."

Review date: 11 Dec 2019 | Direct-zol™ RNA Miniprep Plus