How to extract RNA from hard-to-lyse samples in TRIzol
9 Feb 2023RNA isolation is a challenging procedure. Unlike DNA, which is highly stable, RNA is very unstable, quickly digested by RNase enzymes if their activity isn’t immediately inhibited. One popular reagent in RNA isolation protocols is TRIzol®. TRIzol was specifically formulated to isolate total RNA from any sample type in the modern research laboratory, whether in academia or industry: cells, hard to lyse or solid tissue, and infectious biological samples. In this application, Zymo Research present the best RNA extraction methods for challenging sample types, such as hard-to-lyse samples.
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Zymo-Spin IC Columns
Zymo ResearchCapped version of the Zymo-Spin I column. The Zymo-Spin IC Fast-Spin column can be used in a microcentrifuge for the purification of DNA and/or RNA. The Zymo-Spin IC features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 5 µg DNA or RNA in≥ 6 µl eluate. Capacity is 800 µl.
Direct-zol™ RNA Miniprep Plus
Zymo ResearchThe Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Spin column procedure bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.