Member since: 2020
Organization: avans hogeschool
Great instrument to monitor fluorescently labelled cells
Application Area: Measure GFP and RFP in human cells before and after crispr assays
"In our school we used the cytosmart FL system monitor GFP expression in green. This works very well, and we obtained nice results and we were also able to see how GFP K.O. cells develop after a Crispr experiment. For RFP it was more difficult as we would like to see gain in expression from GFP to RFP but we have no signal to start with, therefore we were not able to set the settings in a most optimal way to start with. However, we were very pleased to monitor our cells for many days in a row."
The CytoSMART Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device enables researchers to unravel cellular processes in real-time, providing lots of valuable data about the experiments and answers if, when, how, and to what extend the cellular processes occur. CytoSMART’s first fluorescence live-cell imager allows users to track dynamic cellular processes with high specificity by taking high-quality images to create real-time time-lapse movies. Simultaneously, the cells are kept in a controlled environment inside a standard cell culture incubator.
The main features and benefits of the CytoSMART Lux3 FL include:
Let Lux3 FL capture fluorescent and brightfield images for you, even when you are not in the lab
Currently, fluorescent labeling is mostly used as an end-point measurement. However, time-lapse imaging of live cells can give much more information about biological processes. By using automated imaging at regular time intervals, the temporal resolution of the fluorescent data is increased, leading to even more relevant data about the cellular processes. In this way, researchers can not only determine if a certain process has occurred, but also when it occurred and at what speed.
Live insight in cellular processes: get the whole story and observe the development of cellular processes while they occur
The CytoSMART Lux3 fluorescence live-cell imaging microscope automatically creates time-lapse movies that contain many cellular features. The videos are made from inside the incubator without disturbing your cells and can be immediately accessed and analyzed remotely via the CytoSMART Cloud, providing real-time updates on your cell cultures and running experiments.
Analyze cells in their desired culture environment: Combine fuorescence and brighflield live-cell imaging inside the same incubator
Kinetic fluorescent imaging of cellular processes requires environmental control throughout the experiment. Because of its small size, the Lux3 FL fits inside any standard incubator. This enables you to perform fluorescent live-cell imaging experiments parallel to your regular cell cultures. Because of the remote data accessibility in the CytoSMART Cloud, your cells do not have to be disturbed during the experiment. This makes sure the cells are in the most optimal culture conditions as possible. Next to this, the remote data accessibility prevents you from making unnecessary lab visits.
Simultaneous analysis of multiple cell features: Expand the number of variables you can analyze in your cell culture
Using CytoSMART Lux3 FL, you can analyze green and red fluorescent tags simultaneously with your brightfield images. This not only saves you time but also provides tools to distinguish cells from each other or distinguish labeled structures within the cells. By tracking changes in the fluorescent signal over time, cell number changes or fluorescently tagged cellular components can easily be identified.
Applications of kinetic fluorescence assays
The use of fluorescence live-cell imaging has become increasingly valuable in the fields of drug discovery, tissue engineering, immunology, immunotherapy, and cancer research. Various applications, such as monitoring cell viability, determining transfection efficiency, investigating co-cultures, and analyzing cellular processes, can benefit from the fluorescent labels and their detailed kinetic visualization. Many of these applications can also benefit from the integrated image analysis included in the CytoSMART Cloud. Imagine effortless calculations of transfection efficiency or the percentage of apoptotic cells.
New feature – Object Count algorithm!
The Lux3 FL, equipped with a new Object Count algorithm, can automatically calculate the number of fluorescent objects in the image and investigate how this number changes over time. What’s more, the existing software interface has become even more intuitive, allowing users to quickly and easily adjust the parameters of an image.