CFSE Cell Division Tracker Kit by BioLegend
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Status:
Reviewer
Member since: 2022
Organization: Pixer
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- Ease of use
- After sales service
- Value for money
Nice product
Application Area: Overall rate
"Awesome and extraordinary product"
Review date: 02 Apr 2022 | CFSE Cell Division Tracker Kit
Status:
Reviewer Member since: 2018
Organization: ONJCRI
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- Ease of use
- After sales service
- Value for money
Great results.
Application Area:T cell proliferation assay
"This product is very effective and it's application is very simple and fast. "
Review date: 13 Dec 2019 | CFSE Cell Division Tracker Kit
CFSE Cell Division Tracker Kit is composed of 5 vials, 100 µg per vial of CFSE (formally known as 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester of CFDA SE), and 500 µl of anhydrous DMSO. CFSE is able to passively diffuse into cells. Inside the cell, its acetate groups are cleaved by intracellular esterases, and the molecules are converted to fluorescent esters. CFSE is retained within the cell and covalently couples to intracellular molecules via its succinimidyl group. Due to this covalent coupling reaction, fluorescent CFSE can be retained within the cell for an extremely long period. Also, due to this stable linkage, once the dye has been incorporated within the cell, it is not transferred to adjacent cells. CFSE is widely used for cell proliferation assays and in vivo cell tracking.
