CellASIC® ONIX2 Microfluidic System

Hypoxia, or inadequate oxygen supply in tissues, is a hallmark of primary tumors, arising due to impaired vascularization and increased respiratory demand of rapidly proliferating cancer cells. This application note presents a study of the hypoxic responses of various cancer cells using the CellASIC® ONIX2 Microfluidic System and assay-optimized plates. These techniques enabled precise control over micro-environmental conditions, including gas and media content and provide results which gave an in depth insight into cancer progression.

This application note uses the CellASIC® ONIX Microfluidic System, in conjunction with the M04S microfluidic plate, to achieve perfusion-based microenvironment control and study drug-induced cell death of 3D cultures of MCF7 cells in Matrigel®. The microfluidic chamber recreates the physiologic mass transport condition for optimized cell health.

This application note demonstrates the application of the CellASIC® ONIX Microincubator Controller for long-term, live cell culture and analysis. It also investigates the speed at which gas conditions could be changed using the microincubator, to assess the utility of the system for studying cell responses to hypoxia.

In this application note the M04S Microfluidic Plate and CellASIC® ONIX Microfluidic System are used to demonstrate long-term culture of adherent cells, to create dynamic solution profiles (media switching and spatial gradient), to immunostain cells within the microfluidic chamber, and to analyze gene expression.

This application note shows how the CellASIC® ONIX Microfluidic System is well suited for host-pathogen studies by providing a stable, long-term culture environment for host cells (including primary cells) with controlled pathogen exposure. The method her demonstrates a host-pathogen experiment using human intestinal cells infected with engineered E. coli strains.

In this application note the multi-parametric microenvironment control capabilities of the CellASIC® ONIX Microfluidic Platform are demonstrated. The successful combination of environment control with perfusion culture in a microfluidic platform promises to further close the gap between in vitro experiments and in vivo relevance.

In this application note the CellASIC® ONIX Microfluidic Platform with M04S plates, is used to develop a dynamic cell-based assay for monitoring the whole autophagy process. This platform offers temperature and gas control as well as media perfusion for precise environmental control within the associated culture chamber.

In this application note the CellASIC® ONIX Microfluidic Plate is used to show that microfluidic control of cell culture conditions and chemoattractant gradient formation enables precise, dynamic quantitation of cell migration in response to stimuli. The results provide a reference point from which to build upon future studies aimed at comparing the effects of signaling molecules and growth factors on the migration propensities of cells in tumors, wounds, developing tissues, immune responses, and other biological systems defined by active cell migration.

This study analyzes the complex relationships within biofilms using the CellASIC® ONIX Microfluidic System. The CellASIC® ONIX microfluidic system is specifically designed to control cell culture parameters and create a stable, manipulatable environment and can be used to conduct long term study of bacterial biofilm dynamics.

In this video, Jun Park, senior scientist at MilliporeSigma, introduces the novel, microfluidic-based live cell imaging platform CellASIC® ONIX2, where microenvironmental parameters such as the perfusion of media/reagent, temperature, and gas compositions can be precisely controlled on-demand via software during the entire imaging experiment.

Techniques enabling live cell analysis with more in vivo-like conditions provide key insights into dynamic processes. In this interview, Dr Amedeo Cappione, Senior Scientist, MilliporeSigma, a business of Merck KGaA, Darmstadt, Germany, explains how the microfluidics-based CellASIC® ONIX2 System offers precise real-time control of media perfusion for cell researchers who need a highly controllable and manipulatable cellular environment and the ability to conduct semi-automated, repeatable long-term experiments while continuously collecting quantitative image-based data.

Philip Lee PhD, Head of Cell Culture Workflow Tools Marketing, introduces MilliporeSigma's CellASIC® ONIX2 microfluidic platform, featuring improved environmental controls. Discover how this versatile system can take your live cell imaging to the next level.

Highlights from an expert presentation on the latest developments in biofilm electrophysiology

New T Cell assays advance insight into immune response

The new technology allows more precise control and manipulation of cell culture environments, converting microscopes into powerful, state-of-the-art tool for live cell imaging

Visit MilliporeSigma at Neuroscience 2016, San Diego, USA, November 12-16, booth 2813

Development of a Novel Cell Based Assay with the Cell ASIC® ONIX2 Platform

Watch interviews from industry experts at this year’s American Association of Cancer Research (AACR) Annual Meeting

New application notes, videos and technologies