EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis.
In contrast to the classical BrdU assays, our EdU-based Cell Proliferation Assays are not antibody based and therefore do not require harsh DNA denaturation for detection of the incorporated EdU. Instead, the EdU-based Cell Proliferation Assays utilize the fast and simple click chemistry for easily coupling fluorophores to the incorporated EdU – allowing a faster and more sensitive detection and quantification in a variety of dye fluorescent readouts.
PromoKine provides three variants of the EdU-based Cell Proliferation Kit III: EdU-FM for fluorescence microscopy, EdU-FC for flow cytometry, and EdU-HTS for fluorometers/fluorescence microplate readers. Each kit variant is available with four different fluorescent labels (green, violet, orange, red) to enable good compatibility and flexibility with other labeling/staining approaches.