Cell Proliferation Kits III (EdU) by PromoCell GmbH

Manufacturer PromoCell GmbH  |  Available Worldwide
Fast and Sensitive Cell Proliferation Assays


Cell Proliferation Kits III (EdU) by PromoCell GmbH product image
Cell Proliferation Kits III (EdU)
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EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis.

In contrast to the classical BrdU assays, our EdU-based Cell Proliferation Assays are not antibody based and therefore do not require harsh DNA denaturation for detection of the incorporated EdU. Instead, the EdU-based Cell Proliferation Assays utilize the fast and simple click chemistry for easily coupling fluorophores to the incorporated EdU – allowing a faster and more sensitive detection and quantification in a variety of dye fluorescent readouts.

PromoKine provides three variants of the EdU-based Cell Proliferation Kit III: EdU-FM for fluorescence microscopy, EdU-FC for flow cytometry, and EdU-HTS for fluorometers/fluorescence microplate readers. Each kit variant is available with four different fluorescent labels (green, violet, orange, red) to enable good compatibility and flexibility with other labeling/staining approaches.

Applications:

  • Cell viability and proliferation assays
  • Cytotoxicity assays
  • Growth factor / cytokine assays
  • Optimization of cell culture conditions

Advantages:

  • Superior alternative to classical BrdU and radioactive [3H]thymidine assays
  • Simple and fast protocol which is completed within only 30 minutes
  • Procedure is gentle to the cell samples - enabling better multiplexing options
  • Optimized for fluorescence microscopy, flow cytometry as well as high-throughput using a fluorescence plate reader.
  • Compatible with multiplexing for content-rich results