BioBLU® 0.3 Single-Use Vessels
As the smallest vessel of the Eppendorf single-use bioreactor family the BioBLU 0.3 is ideally suited for use as a scale-down model. Designed for use with the DASbox Mini Bioreactor System it is able to accelerate process development in biopharmaceutical and biotechnological applications using cell cultures and microorganisms.All critical process parameters can be precisely controlled and users benefit from easy handling, min…
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As the smallest vessel of the Eppendorf single-use bioreactor family the BioBLU 0.3 is ideally suited for use as a scale-down model.
Designed for use with the DASbox Mini Bioreactor System it is able to accelerate process development in biopharmaceutical and biotechnological applications using cell cultures and microorganisms.
All critical process parameters can be precisely controlled and users benefit from easy handling, minimized set-up time and the elimination of cleaning and sterilization steps. The unique liquid-free exhaust gas condenser reduces negative effects caused by change of medium osmolarity and efficiently prevents blocking of the exhaust gas filter.
BioBLU® 0.3 Single-Use Vessels Features:
- Fully instrumented single-use mini bioreactors for accelerated process development in cell culture and microbial applications.
- Excellent scalability and reproducibility.
- Small working volumes starting at 65 mL save on cell material, media and supplements.
- Used with the DASbox mini bioreactor system for parallel set-up of 4, 8, 12 or more bioreactors.
- Liquid-free exhaust condenser with easy handling by automatic click in activation and click out deactivation mode.
- Magnet-coupled overhead drive reduces the risk of contamination, up- or downflow.
- Headplate with 2x PG 13.5 ports, 4x integrated long dip tubes, 2x integrated short dip tubes, one thermowell, DO sensor port with permeable membrane.
- Operating with industry standard probes for precise measurement and control of temperature, pH, DO.
- Vessels are delivered pre-sterilized by ß-irradiation to dose exceeding 15 kGy (DIN EN ISO 11137-2:2007).
- All wetted materials are USP Class VI certified.
Applications:
- Process development in cell culture and microbiology
- Design of Experiments (DoE)
- Media optimization
- Clone & cell line screening, strain characterization
Optimizing CD4+ T cells long-term expansion process in the DASbox Mini Bioreactor System: Impact of the dissolved oxygen
In this application note, Eppendorf tested the suitability of BioBLU® 0.3c Single-Use Bioreactors controlled by a DASbox Mini Bioreactor System in the long-term expansion of CD4+ T cells as well as the impact of different oxygen tensions on cell proliferation.
Microaerobic fermentation within gut physiological conditions
There is growing interest across the food and feed and biofuel industries in microaerobic fermentation, a process occurring at close to anaerobic conditions, but still requiring small amounts of oxygen. This application note describes the microaerobic fermentation of a probiotic strain, Lactobacillus acidophilus, at a very low oxygen level using BioFlo® 120 and 320 control stations.
Controlled, Large-Scale Manufacturing of hiPSC-Derived Cardiomyocytes
In this application note, Eppendorf discusses how functional human cardiomyocytes, derived from a diverse set of disease hiPSC lines, can be manufactured at a large scale using a controlled, stirred-tank bioreactor approach.
Growth of Methanotrophic Bacteria in Chemostat Mode in Eppendorf BioBLU f Single-Use Vessels
To investigate the methanol metabolism of Methylomicrobium alcaliphilum researchers at the San Diego State University analyzed bacterial growth and substrate utilization in chemostat mode. They used a parallel DASbox® Mini Bioreactor System equipped with BioBLU 0.3f Single-Use Vessels. Parallel experimentation using single-use equipment saved time and reduced possible sources of fluctuation between replicates.
Intelligent Control of Chinese Hamster Ovary (CHO) Cell Culture Using the BioFlo® 320 Bioprocess Control Station
In this application note, Chinese Hamster Ovary (CHO) batch cell culture runs were conducted to highlight the versatility of the new BioFlo 320 bioprocess control station, and as such, various sensors and control strategies were employed. The BioFlo 320 combines features and benefits from the New Brunswick™ BioFlo/CelliGen® 310 benchtop, autoclavable bioreactor system and the New Brunswick CelliGen BLU bioreactor to create an all-in-one bioprocess system with unique capabilities for intelligent cell culture. The BioFlo 320 can interchangeably control industry-standard autoclavable glass vessels or BioBLU single-use vessels. In addition to increased versatility with respect to vessels, the BioFlo 320 offers the ability to seamlessly connect a wide variety of Mettler-Toledo ISM sensors.
Scalable Expansion of Human Pluripotent Stem Cells in Eppendorf BioBLU® 0.3 Single-Use Bioreactors
This application note describes the successful cultivation of human pluripotent stem cells in suspension culture using Eppendorf BioBLU 0.3 Single-use Vessels in a DASbox® Parallel Mini Bioreactor System.
Cultivation of Human CAP® Cells: Evaluation of Scale-Down Capabilities using Single-Use Bioreactors
The following application note illustrates how the DASbox® Mini Bioreactor System combined with the BioBLU® 0.3c single-use vessels supports bioprocess development in human cell culture.
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Eppendorf Expands Single-Use Vessel Portfolio for Microbial Fermentation
Eppendorf expands its portfolio of rigid-wall single-use vessels for fermentation
