Member since: 2019
Organization: King's College London
Works for IP. Less sensitive for Western Blot.
Application Area: Immunoprecipiation, Western blot
"It works for IP at the dilution suggested. For Western Blot it's not very powerful. Weak signal, however clean (no background). Needs to be used concentrated (1:250-1:500)."
GA-binding protein alpha chain (UniProt Q00422; also known as GA repeat binding protein alpha, GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor-2) is encoded by the Gabpa gene (also known as E4tf1a) in murine species (Gene ID 14390). GABPalpha is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPalpha is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.