Xevo G2-XS QTof

Desorption electrospray ionisation mass spectrometry is a surface analysis technique that can rapidly analyse the lipid and metabolite composition of a tissue section. By moving the tissue under the analysis probe, a chemical map of the sample can be generated in a matter of minutes. Each tissue type or disease state has its own characteristic molecular fingerprint, therefore, using histologically annotated databases of these molecular profiles, these maps can be classified using machine learning approaches. This poster investigates how this technique could potentially be used to assist in histopathology workflows.

Helium is the most commonly used carrier gas in gas chromatography, however, finite reserves results in periodic price increases and concern of availability. Nitrogen is a more affordable alternative. This application note highlights the ease of implementing nitrogen as a replacement carrier gas for helium in GC separations, illustrating a cost-effective approach for atmospheric pressure MS sources, while maintaining critical separations and chromatographic performance.

Untargeted metabolomics and lipidomics are hypothesis-generating and exploratory in nature, their purpose is to perform an unbiased screening of metabolites and lipids in biological samples, allowing us to determine patterns or variations between different groups: healthy versus diseased, control versus treated or wild-type versus genetically modified.

This application notebook explains the use of novel and unique chromatographic and spectroscopic tools and methods for metabolomic and lipidomic solutions.

RAMMP studies have shown comparable group discrimination and improved selectivity over conventional UPLC chromatography. This application note demonstrates further improvements to the workflow by coupling RAMMP with a novel DIA method (SONAR), providing highly specific and unbiased two-dimensional metabolomic data.

SONAR is an acquisition technique comprised of a low-resolution quadrupole mass filter, which is scanned repetitively and both precursor and MS-MS data are acquired at spectral rates approaching 2000 spectra/s.

SONAR™ is the newest data independent aquisition (DIA) mode from Waters and is complementary to MS. The strength of SONAR is its increased selectivity, which translates to cleaner MS/MS data, allowing both discovery and quantification from a single injection using commonly accepted and established workflows.

SONAR delivers this increased selectivity with rapid scan rates to provide the following analytical benefits:

• All the information required for quantification and identification in a single injection
• Scan rates compatible with UPLC speeds for reliable quantitation
• Library searches are more reliable due to cleaner MS/MS spectra
• Laboratory workflows become more efficient as time consuming method development associated with MS/MS analysis is removed

This white paper discusses in detail the principles and applications of SONAR

Used to extinguish flammable liquid fuels, AFFFs consist of numerous fluorocarbon and hydrocarbon compounds. Tracking these constituents' inadvertent release into the environment is important. This application note demonstrates the use of UNIFI software for easy identification of the markers of interest using a multivariate analysis approach.

A chromatographic peptide mapping method must provide adequate resolution and recovery of a broad set of peptides if it is to be used in the quality analysis of a biotherapeutic protein. In this application note, a range of reversed phase columns by Waters are analysed for their applicability to peptide mapping, demonstrating high peak capacity separations.

Laboratory testing for illicit drug substances frequently employs a combination of immunoassay based screening followed by confirmatory testing using LC-MS/MS techniques. This application note presents a technique for the analysis of 12 common drugs detected in urine using Tof-MRM, utilizing the same well-established chromatographic separation as used for Forensic Toxicology Screening Application Solution with UNIFI.

This poster highlights how mass spectrometric analysis of ex vivo tissue slices from metastatic murine xenograft tumors from LM2-4 cell line with Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) allowed direct comparisons with histology images to determine the molecular profile of necrotic tissues. Showcasing that mass spectrometry is an ideal novel tool for rapid pathology and is highly complementary to current histology based methods widely used in characterization of cancer.

Waters® introduces DESI imaging for tissue identification and characterization in ovarian cancer research using the Xevo® G2-XS QTof mass spectrometer and lipidomic analysis. The use of DESI can help distinguish between several different tissue types in ovarian cancer samples during clinical research.

From desorption electrospray ionization (DESI) for mass spectrometry imaging, to discovery and targeted omics, accelerate your oncology research with the wide range of analytical technologies for characterizing biomolecular signatures of cancer from Waters Corporation.

This interview was filmed as part of our coverage of AACR 2019 - see other video highlights here >>

Unlike conventional mass spec, imaging mass spectrometry enables the construction of molecular images that reveal the spatial resolution of the tissue. In this video, Dr. Bindesh Shrestha, Senior Application Support Scientist at Waters Corporation, explains a fully automated workflow for mass spec imaging from Waters that can process hundreds of samples without human intervention. This high-throughput protocol, as Shrestha describes, has a variety of applications such as tumor tissue classification and 3D mapping of spheroids.

Learn more about mass spec imaging in this downloadable poster.

As biopharmaceutical organizations explore the use of Antibody Drug Conjugates (ADC) as a class of cancer therapies, significant challenges are found in analyzing their complex, heterogeneous structures. The determination of drug-to-antibody ratios (DAR) and sites of conjugation presents unique analytical challenges that can be addressed with Waters' fit-for-purpose UPLC, QTof MS, and informatics solutions.

Michael Batey, Senior Manager Business Development, Waters Corporation, presents a poster by Dr. Zarrine-Afsar, University of Toronto, demonstrating the use of DESI-MSI (Desorption Electrospray Ionization-Mass Spectrometry Imaging) to rapidly detect necrosis in a preclinical model of breast cancer. Speaking at AACR 2017, Michael describes how this technique can identify a biomarker which is specific for necrosis, and its location within the tumor. Michael also presents data which highlights the advantages of DESI-MSI to identify necrosis over traditional histology techniques, such as H&E staining.

Watch this video to find out more about metabolic profiling and its use in analyzing athletic performance at the Rio Olympic Games in 2016.

Find out how scientists at the University of Toronto are using the Waters Xevo G2-XS QTof for the mass spec analysis of tumors.

Discover more in this poster.

Watch this video to learn how the Xevo G2-XS from Waters is essential in the highly sensitive screening of compounds leached from food packaging. Cristina Nerin, Professor of Analytical Chemistry at the University of Zaragoza, Spain, describes how she uses UPLC and the Xevo G2-XS to separate then detect unknown compounds at levels as low as 1ppb.

In this video the ACQUITY UPLC I-Class, Xevo G2-XS QTof and UNIFI Scientific Information System brings the power and versatility of high resolution mass spectrometry to the routine forensic toxicology laboratory.

Learn about the use of QTOF technology for screening toxicology samples for designer drugs. In the absence of known targets, scientists must use non-targeted and semi-targeted screening methods to screen for drug fragments predicted from models of existing designer drugs.

Watch this video to discover how the TOF mass spectrometers from Waters have led to advances in proteomics and metabolomics at Colorado State University. Their robustness and speed allows for non-targeting metabolite identification which has transformed agricultural breeding approaches.

Scientists from Abu Dhabi’s RASID lab provide an insider’s look at the high-throughput wastewater analysis facility

Catch up on some of the most exciting advancements in mass spectrometry presented at ASMS 2022

Enhancements for the Waters Xevo G2-XS QTof, SYNAPT XS and SELECT SERIES Cyclic IMS instruments accelerate biomedical and pharmaceutical research

IMMray™ PanCan-d blood-based test for early detection of pancreatic cancer now set for Q3 2020 launch

We look back at the 2019 American Association for Cancer Research (AACR) annual meeting and the technology set to propel cancer research

Direct sample classification by mass spectrometry speeds creation of test results and decision-making

Prepare for the American Society for Mass Spectrometry(ASMS) Annual Meeting by discovering the many applications of mass spectrometry – from pesticide detection to imaging for cancer therapy

Trace amounts of unknown compounds detected with revolutionary accuracy