VeriSpray™ PaperSpray Ion Source

Chromatographic mass spectrometry methods are considered the gold standard in forensic drug testing due in part to their high sensitivity and selectivity. Unfortunately for many confirmatory tests, extensive sample preparation steps and lengthy run times are required for adequate analysis. Paper spray mass spectrometry (PS-MS) is a potential alternative mass spectrometry method featuring negligible sample preparation, rapid analysis, and adequate sensitivity and selectivity.

This technical note from Thermo Fisher Scientific demonstrates sensitive and accurate quantitation of THC in oral fluid and THC-COOH in urine, through PaperSpray MS with the VeriSpray ion source, while avoiding lengthy sample preparation. It also finds that sensitivity is dramatically increased using Fast Red RC derivatization alongside reactive paper spray mass spectrometry.

Innovation in analytical technologies can enable anti-doping authorities and testing laboratories to stay ahead of evolving doping techniques and illicit substances to ensure a trustworthy and clean sporting environment. Continued enhancement of such technologies, including heightened sensitivity and accelerated processing speeds, plays a pivotal role in shaping the trajectory of future anti-doping endeavors.

In this eBook, we provide information and solutions to ensure you are up to date with the latest advancements in anti-doping technologies. The aim of this guide is to provide you with the cutting-edge tools and methodologies that stand at the forefront of the fight against doping, ensuring a fair and integrity-driven landscape in the world of sports.

Explore the content to:

• Conduct a robust analysis of banned substances in equine plasma
• Utilize an all-in-one LC-MS/MS solution for toxicology
• Stay ahead of regulations for steroid analysis in urine
• Gain unstoppable confidence with user-centric GC-MS/MS systems
• Solve the challenges in your analytical testing labs
• Set the new standard in productivity
• Couple GC-IRMS with quadrupole GC/MS for confirmatory analysis
• Discover a novel technique for the detection of polar compounds

This application note describes a method for quantitative and robust analysis of EDDP (2-ethylidene1,5-dimethyl-3,3-diphenylpyrrolidine), an opioid metabolite, in human whole blood for clinical research using the VeriSpray Paper Spray ion source coupled with a TSQ Quantis mass spectrometer.

The Thermo Scientific™ VeriSpray™ PaperSpray Ion Source is a fully automated PaperSpray ionization solution that is compatible with next-generation Thermo Scientific mass spectrometers. The VeriSpray ion source provides the simplicity of PaperSpray including minimizing sample preparation and avoiding complicated chromatographic separations. The VeriSpray system includes the PaperSpray ion source, capable of running individual proprietary PaperSpray sample plates allowing up to 24 samples per plate, which can also be combined with the plate loader to run multiple plates in a high-throughput environment. For a complete, easy-to-use PaperSpray workflow, the VeriSpray system is seamlessly integrated into the instrument control and application specific software.

Food safety is of paramount importance to public health, and animal products destined for human consumption are closely monitored by global food agencies to ensure that Maximum Residue Limits (MRLs) are not exceeded.

In this veterinary drug residue testing eBook, we consider the Thermo Fisher Scientific suite of LC-MS/MS technologies and their add-ons through a range of case studies demonstrating their use in meat and milk veterinary drug residue testing. We will explore how these analytical methods can tackle specific groups of compounds or high-throughput simultaneous screening of multiple residues, overcoming muscle matrix problems and improving Limits of Quantification (LOQs).

Common concerns with using conventional chromatography-based mass spectrometry (MS) include time-consuming protocols, experiment backlogs, high costs per sample, equipment downtime for maintenance and the need for experienced technicians.

Using paper spray ionization techniques, and the Thermo Scientific™ VeriSpray™ PaperSpray ion source, researchers in clinical and forensic toxicology labs with high-throughput requirements can now perform direct mass spectrometry-based analyses with minimal solvent consumption and sample prep, thereby saving costs per test and reducing the time involved.

In this eBook, we provide case studies and proof-of-concept experiments that corroborate the varied benefits of employing paper spray ionization methods, and cover:

• Detection of controlled substances in blood samples
• Quantitative analysis of antifungal drugs
• Robustness characterization of quantitative whole blood assays
• Quantification of immunosuppressants in whole blood
• Improving the limit of quantification in clinical research
• Analysis of immunosuppressant drugs
• Quantitative analysis of EDDP
• Drugs of abuse screening and quantification

In this new eBook, find cutting-edge technologies to solve anti-doping analysis challenges

Collect our three guides for robust and reliable technologies to enable scientists in forensic and clinical laboratories