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UltraScript™ cDNA Synthesis Kit

PCR BiosystemsPB30.11-02

High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. UltraScript™ cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR.

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The UltraScript™ Reverse Transcriptase, buffer system and optimized blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

Features

  • Unbiased representation of 5’ and 3’ mRNA transcript ends
  • Sensitive detection of low copy number transcripts
  • High cDNA yields from as little as 4pg total RNA
  • Reduced RNase H activity
  • Simple 2 tube system
  • 5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2
  • 20x UltraScript™ RTase is pre-blended with RNase inhibitor

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