Transcreener® UDP² Glycosyltransferase Assays
The Transcreener® UDP² Glycosyltransferase Assay Kit directly detects UDP—the conserved product of glycosyltransferase reactions—in a homogeneous mix-and-read format. Compatible with FP, FI, and TR‑FRET readouts, it supports UDP concentrations from 1–250 µM and delivers exceptional HTS performance (Z’ ≥ 0.7) across 96-, 384-, and 1536‑well formats.
FP Glycosyltransferase Assay
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Nice product. I was able to establish a robust HTS assay with relative ease.
High throughput screening assay
Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.
Review Date: 6 Aug 2019 | BellBrook Labs
A universal, mix-and-read assay for direct fluorescent detection of UDP, the invariant product of glycosyltransferase activity. Designed for HTS, it simplifies workflows while ensuring accurate, reproducible results.
Key Features & Benefits
- Direct UDP Detection – No coupling enzymes required; minimizes false positives.
- Universal Application – Works across glycosyltransferases such as galactosyltransferases, glucuronyltransferases, and UGTs.
- Multiple Detection Modes – Supports FP, FI, and TR-FRET readouts for flexibility.
- High Sensitivity – Detects UDP from 1 to 250 μM with robust Z’ ≥ 0.7.
- HTS-Ready – Validated in 96-, 384-, and 1536-well plate formats.
- Automation-Compatible – Stable signal and homogeneous format for streamlined screening.
Applications
- High-throughput screening of glycosyltransferase inhibitors
- SAR and selectivity profiling
- Substrate specificity and mechanistic studies
Transcreener® High Throughput Screening Assays for Glycosyltransferases
Identifying glycosyltransferase inhibitors remains a challenging task in drug discovery, because for many enzymes in this diverse class, screening methods have been limited to expensive and cumbersome radiolabeled assays or HPLC/MS analyses. As shown here, over the last few years Transcreener® glyscosyltransferase assays have been developed based on the simple and direct immunodetection of nucleotides, which are easily adaptable to automation and systemized high throughput screening protocols.
Screening Diverse Glycosyltransferases Using Homogenous, Fluorescent Transcreener HTS Assays for Direct Detection of Nucleotide Products
Glycosyltransferase enzymes participate in extremely diverse metabolic and regulatory roles by catalyzing the transfer of sugar molecules to protein, lipid and carbohydrate acceptors as well as endogenous and xenobiotic small molecules. From a drug discovery point of view, they are gaining increasing interest as targets for “substrate reduction therapy” in lysosomal storage diseases, and as anti-microbial targets for disrupting bacterial cell wall biosynthesis. This study indicates that a suite of Transcreener assays may be a powerful approach for screening and profiling diverse glycosyltransferases in humans.
