SureSelect Focused Exome
The SureSelect Focused Exome is a highly targeted design that enables analysis of only disease-associated targets, enabling superior coverage of disease-associated regions even on benchtop sequencers. This design provides 20 or more reads for 95% of targets at 1.5Gb (100x) of sequencing and 98% of targets with 3Gb (200x) of sequencing.
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The SureSelect Focused Exome is a highly targeted design that enables analysis of only disease-associated targets, enabling superior coverage of disease-associated regions even on benchtop sequencers. This design provides 20 or more reads for 95% of targets at 1.5Gb (100x) of sequencing and 98% of targets with 3Gb (200x) of sequencing.
Combined with SureSelectQXT, the fastest target enrichment workflow that enables 3.5x faster sample to sequencing compared to other competing solutions, and SureCall, a raw data to variant analysis software, SureSelect Focused Exome supports an efficient workflow that reduces turn-around time from raw data to mutation report.
Features:
- Focused coverage of only disease-associated regions even on a benchtop sequencer
- Highly optimized design for deep coverage of targets in HGMD, OMIM, and ClinVar, 98% at 20x, for highly sensitive variant detection
- Sample to sequencing in a day, when complemented by the efficient SureSelect QXT workflow with a novel 90-minute hybridization step for faster time to sequencing
- Complete and flexible solutions for library prep, enrichment, QC, and analysis enables a full workflow solution for target analysis using high output or benchtop sequencers
For Research Use Only. Not for use in diagnostic procedures.
Targeted Exome and Gene Panel Analysis from Low Input and FFPE DNA using Hybridization Capture for Cancer Genome Studies
Hybridization-based target enrichment techniques coupled with Next-Generation Sequencing (NGS) provide a useful and cost-efficient means to study disease specific target regions including whole exomes and gene panels. Clinical samples may be limited in input and of compromised quality due to formalin fixation, however most current NGS library preparation methods require 50-100 ng high quality DNA for such studies. This application note presents an efficient method, which enables high quality target enrichment and variant calling from inputs as low as 1-25 ng.
