Seahorse XF T Cell Metabolic Profiling Kit
The Agilent Seahorse XF T Cell Metabolic Profiling kit allows for robust and accurate measurements of both glycolytic and mitochondrial activities in T cell populations, providing a complete picture of T cell energy metabolism. These measurements can be linked to antitumor properties of T cell therapy products and are therefore valuable in designing and optimizing therapy development processes to improve T cell persistence or…
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The Agilent Seahorse XF T Cell Metabolic Profiling kit allows for robust and accurate measurements of both glycolytic and mitochondrial activities in T cell populations, providing a complete picture of T cell energy metabolism. These measurements can be linked to antitumor properties of T cell therapy products and are therefore valuable in designing and optimizing therapy development processes to improve T cell persistence or avoid exhaustion in the tumor microenvironment. The kit not only provides improved reagents, but also features a streamlined assay workflow, reducing assay preparation time and minimizing the need for uncoupler optimization. The XF T Cell Metabolic Profiling kit is also integrated with Wave Pro and Seahorse Analytics software to simplify data analysis, visualization, and interpretation.
Each assay kit contains sufficient materials for six full-plate tests and is available in two packaging sizes. The XF kit is for use with Agilent Seahorse XF Pro and XFe/XF96 analyzers. The XFp kit is for use with the Agilent Seahorse HS Mini and XFp analyzers.
Assessing T cell bioenergetic poise and spare respiratory capacity using extracellular flux analysis
In this application note, Agilent Technologies presents the Agilent Seahorse XF T Cell Metabolic Profiling kit, a robust solution for real-time assessment of T cell metabolism. It measures glycolytic and mitochondrial activity along with respiratory capacity, enhancing T cell therapy efficacy. The kit uses an improved uncoupler (BAM15) for accurate measurements without extensive concentration optimization. It also evaluates medium composition during T cell expansion, providing a comprehensive metabolic profile for therapy improvement.
Analyzing T-Cell metabolism using the XF T cell metabolic profiling kit and Seahorse Analytics
In this video, Agilent Technologies showcases its XF T cell metabolic profiling kit and two tailored assays, the XF T cell Persistence Assay and XF T Cell Fitness assay. Using Seahorse Analytics, Agilent demonstrates how to analyze data from these assays. The XF T cell Persistence assay evaluates T cell memory-like phenotype, while the XF T Cell Fitness assay assesses bioenergetic capacity in tumor microenvironment-like conditions. Through spare respiratory capacity and ATP production rate analysis, they identify differences in metabolic profiles between IL-2 and IL-15 expanded cells. The video aims to aid researchers in understanding T cell metabolism for T cell therapy development.
