QuantiFluor® dsDNA System
The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The…
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It is my first choice when it comes to DNA quantification
DNA Quantification
Very accurate and reliable. I tried many other kits and system and this is the best.
Review Date: 15 Nov 2021 | Promega Corp.
Easy to use, fast, sensitive.
Plasimd DNA quantification
Easy to use, fast results. Very sensitive compared to the UV without interference from protein and RNA. The kit is big enough for large number of tests.
Review Date: 11 Jan 2017 | Promega Corp.
The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.
The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.
The QuantiFluor® dsDNA System was developed using the fluorescence module of the GloMax® Multi+ Detection System and the QuantiFluor®-ST Handheld Fluorometer. The QuantiFluor® dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths, including the Quantus™ Fluorometer.
Each QuantiFluor® dsDNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl.
QuantiFluor® dsDNA System Features & Benefits:
- Specificity - Highly specific to dsDNA, minimal binding to ssDNA, RNA, protein and interfering compounds.
- Sensitivity - Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye and can detect as little as 50pg/ml.
- Ease of Use - System includes all required reagents to quickly set up and quantitate dsDNA.
- Instrument Compatibility - Pre-optimized on both the Quantus™ Fluorometer and GloMax®-Multi+ Instrument.
DNA Quantitation in Next-Generation Sequencing Library Workflows
The rapid advancement of next-generation (NGS) technology has impacted all branches of life science research. For any NGS workflow, the success of the NGS depends to a large extent on using high-quality starting DNA that is quantitated accurately. This article discusses the role of DNA quantitation during typical NGS library preparation workflows.
Correlation of Mutations Detected in Liquid and Tissue Biopsies Using the Maxwell® RSC
This application note describes a method for purifying nucleic acids using the Maxwell® RSC instrument, based on novel surface and binding chemistries. Circulating cell-free DNA (ccfDNA) in plasma can be used to detect biomarkers that show great promise for diagnosis and monitoring of cancer, giving rise to the possibility of liquid biopsies that obviate the need for invasive tissue collection. This protocol is completely automated, decreasing the chance of user error or contamination and sixteen samples of up to 1ml can be processed in 80 minutes, making next generation sequencing of DNA a viable potential method for screening plasma in a form of liquid biopsy.
Quantitating DNA from FFPE Tissue: Superior sensitivity of the QuantiFluor dsDNA System versus the NanoDrop Spectrophotometer
Accurate quantitation of DNA concentration is critical for many applications. Traditional spectrophotometric assays cannot determine DNA concentrations below 2 ug/ml; however, many isolated DNA samples have concentrations well below this level. This study comparatively examines the performance of the QuantiFluor™ dsDNA System with regards to accuracy and sensitivity to sample concentrations.
